Department of Information and Communication Technologies in Medicine, Faculty of Biomedical Engineering, Czech Technical University in Prague, Kladno, Czech Republic.
Physiol Res. 2019 Aug 29;68(4):611-622. doi: 10.33549/physiolres.933998. Epub 2019 Jun 6.
Effects of low-frequency electromagnetic fields (LF EMF) on the activation of different tissue recovery processes have not yet been fully understood. The detailed quantification of LF EMF effects on the angiogenesis were analysed in our experiments by using cultured human and mouse endothelial cells. Two types of fields were used in the tests as follows: the LF EMF with rectangular pulses, 340-microsecond mode at a frequency of 72 Hz and peak intensity 4 mT, and the LF EMF with sinusoidal alternating waveform 5 000 Hz, amplitude-modulated by means of a special interference spectrum mode set to a frequency linear sweep from 1 to 100 Hz for 6 s and from 100 Hz to 1 Hz return also for 6 s, swing period of 12 second. Basic parameters of cultured cells measured after the LF EMF stimulus were viability and proliferation acceleration. Both types of endothelial cells (mouse and human ones) displayed significant changes in the proliferation after the application of the LF EMF under conditions of a rectangular pulse mode. Based on the results, another test of the stimulation on a more complex endothelial-fibroblast coculture model will be the future step of the investigation.
低频电磁场（LF EMF）对不同组织修复过程的激活作用尚未被完全理解。我们通过培养的人源和鼠源血管内皮细胞，对 LF EMF 促进血管生成的效应进行了详细的定量分析。在实验中使用了两种类型的场：一种是矩形脉冲 LF EMF，频率为 72 Hz，脉宽 340 微秒，峰值强度为 4 mT；另一种是正弦波交变电磁场，频率为 5 000 Hz，通过特殊的干扰谱模式进行幅度调制，设定频率线性扫描从 1 Hz 到 100 Hz 持续 6 s，再从 100 Hz 返回到 1 Hz 持续 6 s，摆幅周期为 12 秒。LF EMF 刺激后测量的培养细胞的基本参数包括细胞活力和增殖加速。在矩形脉冲模式下，两种类型的内皮细胞（鼠源和人源）的增殖都发生了显著变化。基于这些结果，未来的研究步骤将是在更复杂的内皮-成纤维细胞共培养模型上进行刺激测试。
