US Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center Molecular Characterization of Foodborne Pathogens Research Unit, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA.
Can J Microbiol. 2020 Apr;66(4):303-312. doi: 10.1139/cjm-2019-0502. Epub 2020 Mar 2.
Herein we describe a highly structured, filamentous growth phenotype displayed by an isolate of the food spoilage microorganism . The growth morphology of this strain (strain BII) was dependent on environmental factors such as the growth media, incubation temperatures, and the inoculum concentration. Inoculation of cultures in highly dilute suspensions resulted in the formation of isolated, tight aggregates resembling fungal growth in liquid media. This same strain also formed stable, mesh-like structures in 6-well tissue culture plates under specific growth conditions. The complex growth phenotype does not appear to be unique to strain BII but was common among strains isolated from chicken. Light and electron micrographs showed that the filaments of multiple BII cells can organize into complex, tertiary structures resembling multistranded cables. Time-lapse microscopy was employed to monitor the development of such aggregates over 18 h and revealed growth originating from short filaments into compact ball-like clusters that appeared fuzzy due to protruding filaments or cables. This report is the first to document this complex filamentous growth phenotype in a wild-type bacterial isolate of .
在此，我们描述了食品腐败微生物 的一个分离株表现出的高度结构化的丝状生长表型。该菌株（BII 株）的生长形态取决于环境因素，如生长培养基、孵育温度和接种浓度。在高度稀释的悬浮液中接种会导致形成类似于液体培养基中真菌生长的孤立、紧密的聚集物。在特定的生长条件下，同一菌株在 6 孔组织培养板中也形成稳定的网状结构。这种复杂的生长表型似乎不是 BII 株所特有的，而是从鸡肉中分离出的菌株的共同特征。光和电子显微镜照片显示，多个 BII 细胞的丝状结构可以组织成类似于多股电缆的复杂三级结构。延时显微镜用于监测这些聚集物在 18 小时内的发育情况，显示出由短丝形成的紧凑球状簇的生长，由于突出的丝或电缆，这些簇呈现出模糊的外观。本报告首次记录了野生型 细菌分离株的这种复杂丝状生长表型。
