一种用钙荧光白检测嗜肝艾美耳球虫(EHP)孢子的染色方法。

A staining method for detection of Enterocytozoon hepatopenaei (EHP) spores with calcofluor white.

机构信息

Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture and Rural Affairs, Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.

Shanghai Ocean University, Shanghai 201306, China; Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture and Rural Affairs, Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.

出版信息

J Invertebr Pathol. 2020 May;172:107347. doi: 10.1016/j.jip.2020.107347. Epub 2020 Feb 28.

DOI:10.1016/j.jip.2020.107347

PMID:32119955

Abstract

A fluorescent stain, calcofluor white (CFW), was used for detection of spores of the microsporidium Enterocytozoon hepatopenaei (EHP). EHP spores in suspension, in feces, or in the infected hepatopancreas of the shrimp Penaeus vannamei, can be easily stained with this chitin and cellulose binding dye to show distinct blue-white fluorescent oval walls. The dye does not stain the host tissues. EHP spores showed orange-red spots by staining with hematoxylin and phloxine (H&P) in the section. CFW staining provides a simple and rapid method for determining the presence of EHP spores in fecal or tissue samples.

摘要

荧光染色剂钙荧光白（CFW）被用于检测微孢子虫的孢子Enterocytozoon hepatopenaei（EHP）。悬浮液中的 EHP 孢子、粪便中的 EHP 孢子或感染的南美白对虾肝胰腺中的 EHP 孢子，都可以用这种结合几丁质和纤维素的染料轻松染色，从而显示出明显的蓝白色荧光椭圆形壁。这种染料不会染色宿主组织。EHP 孢子在切片中用苏木精和曙红（H&P）染色后呈现出橙红色斑点。CFW 染色为确定粪便或组织样本中 EHP 孢子的存在提供了一种简单快速的方法。