使用一种具有侵袭性但非致死性的采样方法检测虾（凡纳滨对虾）中的肠胞虫。

Detection of Enterocytozoon hepatopenaei using an invasive but non-lethal sampling method in shrimp (Penaeus vannamei).

机构信息

Aquaculture Pathology Laboratory, School of Animal and Comparative Biomedical Sciences, The University of Arizona, Tucson, AZ, USA.

出版信息

J Microbiol Methods. 2019 Jul;162:38-41. doi: 10.1016/j.mimet.2019.05.008. Epub 2019 May 17.

DOI:10.1016/j.mimet.2019.05.008

PMID:31103459

Abstract

The detection of enteric pathogens that cause diseases in shrimp involves the sacrifice of the host to obtain tissue samples for diagnosis. In this study, we describe an invasive but non-lethal sampling methodology using a syringe to collect biopsy samples from the hepatopancreas (HP) of Penaeus vannamei to detect the microsporidian pathogen, Enterocytozoon hepatopenaei (EHP), by qPCR and transmission electron microscopy. EHP was detected in all the infected shrimp by qPCR. The shrimp infected by the microsporidian showed 65% survival at 7 days post-sampling. Transmission electron microscopic examination of the biopsy samples revealed numerous spores of the pathogen. The presence of EHP was further confirmed by histology and in situ hybridization from HP tissue samples. The data shows that a hepatopancreas biopsy could be a viable means of detecting enteric pathogens in shrimp, and the method could be valuable in sampling broodstock and natural populations without the need to sacrifice the animals.

摘要

检测引起虾类疾病的肠道病原体需要牺牲宿主以获取组织样本进行诊断。在本研究中，我们描述了一种侵袭性但非致死性的采样方法，使用注射器从凡纳滨对虾的肝胰腺（HP）采集活检样本，通过 qPCR 和透射电子显微镜检测微孢子虫病原体，对虾微孢子虫（EHP）。qPCR 检测到所有受感染的虾中均存在 EHP。感染微孢子虫的虾在采样后 7 天的存活率为 65%。对活检样本的透射电子显微镜检查显示出大量病原体的孢子。HP 组织样本的组织学和原位杂交进一步证实了 EHP 的存在。数据表明，肝胰腺活检可能是一种可行的方法，可以检测虾类的肠道病原体，并且该方法在采样亲虾和自然种群时无需牺牲动物，可能具有重要价值。