Department of Gynecology and Obstetrics, The Third Xiangya Hospital, Central South University, Changsha, 410013, Hunan, China.
Department of Gynecology and Obstetrics, The Second Hospital of Zhuzhou, Zhuzhou, 412000, Hunan, China.
Biotechnol Lett. 2020 Jun;42(6):1051-1060. doi: 10.1007/s10529-020-02847-8. Epub 2020 Mar 2.
This study on all-trans retinoic acid was designed to explore its effect on the ability of Fra-1 to cervical cancer cell development. The results show that all-trans retinoic acid enhances the effect of Fra-1 on inhibiting cervical cancer proliferation and the glucose consumption, its effect on the loss of mitochondrial membrane potential, on the decreasing of lactic acid as well as ATP, and also influences the expression of MDM2/P53/P21 and LDHA.
The results show that the expression of Fra-1 is higher in all-trans retinoic acid-treated cervical cancer. Flow cytometry and kit detection show that all-trans retinoic acid can enhance the ability of Fra-1 to lose the mitochondrial membrane potential, inhibit the glucose consumption and the production of lactic acid as well as ATP. CCK8 and colony formation assays indicate that all-trans retinoic acid enhances the ability of Fra-1 to inhibit cell proliferation. In addition, through Western blot analysis, it was determined that P53 and P21 were up-regulated, and MDM2 and LDHA were down-regulated.
The overall results of the study strongly suggest that all-trans retinoic acid enhances the effect of Fra-1 on inhibiting cervical cancer proliferation and metabolism in vitro, and also influences the expression of MDM2/P53/P21 and LDHA.
本研究旨在探讨全反式维甲酸对 Fra-1 抑制宫颈癌发展能力的影响。结果表明，全反式维甲酸增强了 Fra-1 抑制宫颈癌增殖和葡萄糖消耗的作用，其对线粒体膜电位丧失、乳酸和 ATP 生成减少的影响，以及对 MDM2/P53/P21 和 LDHA 的表达的影响。
流式细胞术和试剂盒检测结果表明，全反式维甲酸可增强 Fra-1 丧失线粒体膜电位的能力，抑制葡萄糖消耗和乳酸及 ATP 的产生。CCK8 和集落形成实验表明，全反式维甲酸增强了 Fra-1 抑制细胞增殖的能力。此外，通过 Western blot 分析，确定 P53 和 P21 上调，MDM2 和 LDHA 下调。
综上所述，本研究结果强烈提示，全反式维甲酸增强了 Fra-1 抑制体外宫颈癌增殖和代谢的作用，并影响了 MDM2/P53/P21 和 LDHA 的表达。
