Sato R
Hokkaido Igaku Zasshi. 1978 Jul;53(4):287-303.
The effects of acute and chronic administration of D-Galactosamine (GalN), Ethanol and Phenobarbital were investigated on the activities of lysosomal enzymes, i.e.; acid phosphatase, beta-glucuronidase and n-acetyl-beta-glucosaminidase, and others such as gamma-GTP and adenosine triphosphatase. The histochemical distribution of gamma-GTP in the liver was also studied on biopsy specimens from patients with chronic hepatitis, and gamma-GTP levels in the serum of patients receiving drugs inductable of hepatic microsomal enzymes. 1) After a single intraperitoneal injection of GalN, the lysosomal enzyme activities were lowered in the necrotic areas, but raised in the perinecrotic areas, the proliferative Kupffer cells and intra- and/or extra-cellular eosine bodies. 2) gamma-GTP activities in rat liver after chronic administration of GalN were markedly increased in bile canalicular membrane of periportal parenchymal cells, the epithelium of bile duct and ductules, and som inflammatory cells of portal fields. Levels of serum gamma-GTP were also elevated. On histochemical studies with biopsy specimens from patients with chronic active hepatitis showing elevated gamma-GTP activity, the activity was revealed a similar localization to GalN-treated rats. These data suggested that the increased activities might be reflected on the active stage in chronic hepatitis. 3) Chronic ethanol treatment in rats induced clearly-stained lysosomes varied in size, especially large-sized. The activities of hepatic gamma-GTP were slightly increased in the bile canalicular membrane of periportal parenchymal cells and the epithelium of proliferative bile ductules. 4) It has been shown by histochemical and biochemical techniques that hepatic gamma-GTP activity was increased after phenobarbital administration in rats. A significant rise in serum gamma-GTP was observed in patients on long-term treatment with anti-epileptic drugs. These data indicated that the increased activities of serum gamma-GTP might be accompanied with induction of hepatic microsomal drug-metabolizing enzymes.
研究了急性和慢性给予D-半乳糖胺（GalN）、乙醇和苯巴比妥对溶酶体酶活性的影响，即酸性磷酸酶、β-葡萄糖醛酸酶和N-乙酰-β-氨基葡萄糖苷酶，以及其他酶如γ-谷氨酰转肽酶（γ-GTP）和三磷酸腺苷酶的活性。还对慢性肝炎患者的活检标本进行了肝内γ-GTP的组织化学分布研究，并检测了接受可诱导肝微粒体酶药物治疗患者血清中的γ-GTP水平。1）单次腹腔注射GalN后，坏死区域的溶酶体酶活性降低，但坏死周边区域、增殖的库普弗细胞以及细胞内和/或细胞外嗜酸性小体中的酶活性升高。2）慢性给予GalN后，大鼠肝脏中γ-GTP活性在门周实质细胞的胆小管膜、胆管和小胆管上皮以及门管区的一些炎症细胞中显著增加。血清γ-GTP水平也升高。对γ-GTP活性升高的慢性活动性肝炎患者的活检标本进行组织化学研究发现，其活性定位与GalN处理的大鼠相似。这些数据表明，活性增加可能反映了慢性肝炎的活动期。3）大鼠慢性乙醇处理诱导出大小不一、染色清晰的溶酶体，尤其是大尺寸的。肝γ-GTP活性在门周实质细胞的胆小管膜和增殖小胆管上皮中略有增加。4）组织化学和生化技术表明，大鼠给予苯巴比妥后肝γ-GTP活性增加。长期接受抗癫痫药物治疗的患者血清γ-GTP显著升高。这些数据表明，血清γ-GTP活性增加可能与肝微粒体药物代谢酶的诱导有关。
