Food Safety Laboratory, College of Food Science and Engineering, Ocean University of China, 5 Yushan Rd., Qingdao, China.
Shandong Analysis and Test Centre, Qilu University of Technology (Shandong Academy of Sciences), 19 Keyuan Rd, Jinan, China.
Anal Biochem. 2020 May 15;597:113669. doi: 10.1016/j.ab.2020.113669. Epub 2020 Feb 29.
Lambda-cyhalothrin is a pyrethroid widely used in crop, fruit and vegetable production, but has potential health threats to human. Immunoassay is a cheap, rapid and facile method to detect lambda-cyhalothrin, yet wide application of this method still requires improvement in the construction of antigen. In this study, we developed a one-step lambda-cyhalothrin hapten synthesis that transformed the cyanide group in lambda-cyhalothrin to amide. Complete antigen was assembled by coupling the amide with succinic-anhydride-activated carrier proteins, and corresponding polyclonal antibodies were generated using Balb/c mice. Using antibody generated by the method in this paper, the competitive ELISA demonstrated the lowest detection limit of 3.772 μg/L for lambda-cyhalothrin, and no significant cross-reactivity for other pyrethroid pesticides was observed. All the results suggested we have established a more efficient technique of generating lambda-cyhalothrin antibody. Furthermore, since the activated proteins used in this study are highly controllable, we believe these proteins could potentially be the prototype of a series of standardized carrier proteins for the synthesis of complete antigens.
高效合成拟除虫菊酯类农药敌百虫半抗原及其多克隆抗体的制备
