Garcez A S, Barros L C, Fernandes M R U, Fujii D N, Suzuki S S, Nepomuceno R
Dep. of Orthodontics, São Leopoldo Mandic Dental Institute and Research Center, Brazil; Dep. of Oral Microbiology, São Leopoldo Mandic Dental Institute and Research Center, Brazil.
Dep. of Orthodontics, São Leopoldo Mandic Dental Institute and Research Center, Brazil.
Photodiagnosis Photodyn Ther. 2020 Jun;30:101707. doi: 10.1016/j.pdpdt.2020.101707. Epub 2020 Feb 29.
Peri-miniscrew inflammation is one of the causes of orthodontic miniscrew failure.
The aim of this study was to correlate and quantify throughout autofluorescence images, PCR and microbiologic count of biofilm retained around orthodontic miniscrew and the presence of Porphyromonas gingivalis.
Forty miniscrews used for orthodontic treatment were evaluated during orthodontic treatment, collected from patients and divided into two groups: healthy and inflamed miniscrews. To be considered inflamed, the samples should present: loss of stability checked by periotest®, clinical presence of mucositis, red aspect of the gum or bleeding around the miniscrew. Immediately after removal of the miniscrews, they were photographed using a macro 100 lens and a Pentax camera coupled to a fluorescent equipment - Qscan (AioBio - Korea) with 405 nm excitation wavelength and a blue band filter. A microbiologic sample was collected with a sterile microbrush scrubbed around the miniscrew tread. Fluorescent images were analyzed with ImageJ software to quantify fluorescent intensity and fluorescent area and microbiological samples were submitted to CFU count for total contamination and q-PCR assay to quantify Porphyromonas gingivalis.
The results showed a good correlation between CFU count and fluorescent intensity and PCR/fluorescent area. The healthy miniscrews presented less fluorescent intensity and lower CFU count when compared to inflamed miniscrews. q-PCR analysis showed a higher number of P. gingivalis contamination around inflamed miniscrews.
Quantification of biofilm retained by miniscrew by images of autofluorescence is a simple and reliable method with great potential for clinical use to monitory inflammation around miniscrew and risk of loss.
微型螺钉周围炎症是正畸微型螺钉失败的原因之一。
本研究的目的是通过对正畸微型螺钉周围留存生物膜的自发荧光图像、聚合酶链反应（PCR）和微生物计数以及牙龈卟啉单胞菌的存在情况进行关联和量化分析。
在正畸治疗期间对40颗用于正畸治疗的微型螺钉进行评估，从患者口中收集并分为两组：健康微型螺钉和发炎微型螺钉。若样本出现以下情况则被视为发炎：通过牙周探针检查稳定性丧失、存在黏膜炎的临床表现、牙龈发红或微型螺钉周围出血。微型螺钉取出后，立即使用100微距镜头和与荧光设备-Qscan（韩国AioBio公司）相连的宾得相机进行拍照，激发波长为405nm，配备蓝色滤光片。用无菌微型刷在微型螺钉螺纹周围刷洗收集微生物样本。使用ImageJ软件分析荧光图像以量化荧光强度和荧光面积，微生物样本进行菌落形成单位（CFU）计数以检测总污染情况，并进行定量聚合酶链反应（q-PCR）分析以量化牙龈卟啉单胞菌。
结果显示CFU计数与荧光强度以及PCR/荧光面积之间具有良好的相关性。与发炎的微型螺钉相比，健康微型螺钉的荧光强度较低且CFU计数也较低。q-PCR分析显示发炎微型螺钉周围牙龈卟啉单胞菌的污染数量更多。
通过自发荧光图像对微型螺钉留存生物膜进行量化分析是一种简单可靠的方法，在临床上监测微型螺钉周围炎症及脱落风险方面具有很大的应用潜力。
