Monga Nitika, Chaurasia Sushma, Kharbanda Om Prakash, Duggal Ritu, Rajeswari Moganty Raja
Division of Orthodontics and Dentofacial Deformities, Centre for Dental Education and Research, All India Institute of Medical Sciences, New Delhi 110029, India.
Prog Orthod. 2014 Apr 1;15(1):30. doi: 10.1186/s40510-014-0030-4.
This study provides a vital insight in assessing the clinical and biochemical changes in interleukin (IL)-1β levels in peri-miniscrew crevicular fluid (PMCF) during the course of orthodontic tooth movement.
The study comprised the analysis of IL-1β in peri-miniscrew crevicular fluid obtained from crevices around the miniscrews inserted in 11 patients (eight females and three males, mean age 17.3 ± 4.64 years) with all first premolar extraction and maximum anchorage requirement using miniscrew-supported anchorage. Miniscrews were loaded at 3 weeks after placement by 200-g nitinol closed coil springs of 9-mm length for en masse retraction. Peri-miniscrew crevicular fluid was collected at miniscrew placement (T1), at 3 weeks (T2/baseline) and on loading at 0 (T3) and 1 day (T4), 21 (T5), 72 (T6), 120 (T7), 180 (T8) and 300 (T9) days. IL-1β levels were estimated by enzyme-linked immunosorbent assay (ELISA). Peri-miniscrew tissue was examined for signs of inflammation, and also, miniscrew mobility was assessed with Periotest and handles of two mouth mirrors.
IL-1β levels in all miniscrews were significantly higher at T1 and peaked again at T4 showing a bimodal peak. However, there was a gradual and statistically significant decrease in IL-1β till T5, while further changes till the end of the study were statistically not significant.
The changing levels of IL-1β levels in PMCF over a duration of 300 days are suggestive of the underlying inflammatory process. IL-1β levels in PMCF show a significant rise during miniscrew insertion and on immediate loading. The trend of gradually reducing IL-1β levels around the miniscrew over the period after loading towards baseline is suggestive of adaptive bone response to stimulus.
本研究为评估正畸牙齿移动过程中微型螺钉周围龈沟液（PMCF）中白细胞介素（IL）-1β水平的临床和生化变化提供了重要见解。
本研究分析了从11例患者（8名女性和3名男性，平均年龄17.3±4.64岁）中插入的微型螺钉周围缝隙中获取的微型螺钉周围龈沟液中的IL-1β。这些患者均拔除了第一前磨牙，并使用微型螺钉支持的支抗，对支抗的需求最大。微型螺钉在植入3周后通过长度为9毫米的200克镍钛合金闭合螺旋弹簧加载，以进行整体后移。在微型螺钉植入时（T1）、3周时（T2/基线）、加载时（T3）、加载后1天（T4）、21天（T5）、72天（T6）、120天（T7）、180天（T8）和300天（T9）收集微型螺钉周围龈沟液。通过酶联免疫吸附测定（ELISA）估计IL-1β水平。检查微型螺钉周围组织的炎症迹象，并使用Periotest和两个口镜的手柄评估微型螺钉的松动度。
所有微型螺钉中的IL-1β水平在T1时显著升高，并在T4时再次达到峰值，呈现双峰峰值。然而，直到T5，IL-1β水平逐渐且有统计学意义地下降，而直到研究结束的进一步变化在统计学上不显著。
在300天的时间内，PMCF中IL-1β水平的变化提示了潜在的炎症过程。PMCF中的IL-1β水平在微型螺钉植入时和立即加载时显著升高。加载后一段时间内微型螺钉周围IL-1β水平逐渐降低至基线的趋势提示了骨对刺激的适应性反应。
