Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei 430072, China.
State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China.
Anal Chem. 2020 Mar 17;92(6):4177-4181. doi: 10.1021/acs.analchem.0c00634. Epub 2020 Mar 6.
A clear elucidation of a disease-related viscosity change in vivo is significant yet highly challenging as well. Fluorescence imaging in the second near-infrared region (NIR-II, 1000-1700 nm) has gained increasing attention for observation in living organisms, but a viscosity-activatable fluorescent probe emitting at this region remains a vacancy. Herein, we report the first panel of a viscosity-activated NIR-II emissive fluorescent probe . By embedding different substituents into the platform and screening, we obtained an ideal probe, , which displayed the best combination of properties, including a 31-fold fluorescence enhancement in response to viscosity, insensitivity to environments (pH, polarity), and relatively high quantum yield (1.6% in glycerol). was successfully applied to track the variation of viscosity in diabetes-induced liver injury in vivo.
清楚阐明与疾病相关的体内黏度变化具有重要意义，但也极具挑战性。近红外二区（NIR-II，1000-1700nm）的荧光成像是活体观察中越来越受到关注的一种技术，但在该区域发射的黏度激活型荧光探针仍然是一个空缺。在此，我们报道了第一个 NIR-II 发射型黏度激活荧光探针家族。通过将不同取代基嵌入到 平台中并进行筛选，我们得到了一个理想的探针 ，它具有最佳的性能组合，包括对黏度响应时荧光增强 31 倍、对环境（pH 值、极性）不敏感，以及相对较高的量子产率（在甘油中为 1.6%）。 成功地应用于跟踪糖尿病诱导的肝损伤中体内黏度的变化。
