α-硫辛酸对雌性大鼠 4-乙烯基环己烯二恶烷诱导的原发性卵巢功能衰竭的保护作用。
Protective effect of alpha lipoic acid on 4-vinylcyclohexene diepoxide induced primary ovarian failure in female rats.
机构信息
Dokuz Eylül University, Faculty of Medicine, Department of Histology and Embryology, Izmir, Turkey.
Dokuz Eylül University, Faculty of Medicine, Department of Physiology, Izmir, Turkey.
出版信息
Taiwan J Obstet Gynecol. 2020 Mar;59(2):293-300. doi: 10.1016/j.tjog.2020.01.020.
OBJECTIVE
The effects of alpha lipoic acid (ALA) and its possible mechanisms in treating Primary ovarian failure (POF) model was studied with 4 vinylcyclohexene diepoxide (VCD).
MATERIAL AND METHODS
Rats were divided into 4 groups (n = 7) as Control, VCD, VCD + ALA and ALA. POF model was induced by applying VCD intraperitoneally and ALA was administered by oral gavage as 100 mg/day to the VCD + ALA and ALA groups.
RESULTS
At the end of 42 days, ovarian and uterine tissues were received. The number of primordial and primary follicles were increased and corpus luteum and cystic follicles were decreased in ovarian tissues in VCD + ALA group compared to VCD group. Caspase-3 immunoreactivity in follicular cells was decreased in VCD + ALA group compared to VCD group. eNOS immunoreactivity and eNOS levels were decreased in VCD group and increased in VCD + ALA group while iNOS immunoreactivity and iNOS levels were increased in VCD group, decreased in VCD + ALA group in ovary and uterine tissue. Plasma FSH and LH hormone levels were increased in the VCD but decreased in VCD + ALA group. Estradiol level decreased in the VCD group compared to the other groups. The MDA values were significantly increased in the VCD + ALA group compared to VCD group. In addition, the levels of GSH values were decreased in VCD + ALA group compared to VCD group.
CONCLUSION
Alpha lipoic acid treatment of rats with VCD-induced POF had a beneficial effect on reducing ovarian damage by improving histological, immunohistochemical, hormone level and oxidative stress markers. Our results show that ALA is an effective treatment of VCD-induced POF rats.
目的
用 4-乙烯环己烯二氧化物（VCD）研究α-硫辛酸（ALA）及其可能的机制对原发性卵巢衰竭（POF）模型的治疗作用。
材料和方法
将大鼠分为 4 组（n=7），分别为对照组、VCD 组、VCD+ALA 组和 ALA 组。通过腹腔内注射 VCD 诱导 POF 模型，VCD+ALA 组和 ALA 组每天灌胃给予 ALA100mg。
结果
在第 42 天，取卵巢和子宫组织。与 VCD 组相比，VCD+ALA 组卵巢组织中原始和初级卵泡数量增加，黄体和囊状卵泡减少。与 VCD 组相比，VCD+ALA 组卵泡细胞中 caspase-3 免疫反应性降低。VCD 组卵巢和子宫组织中 eNOS 免疫反应性和 eNOS 水平降低，VCD+ALA 组增加，而 iNOS 免疫反应性和 iNOS 水平在 VCD 组增加，在 VCD+ALA 组降低。VCD 组血浆 FSH 和 LH 激素水平升高，VCD+ALA 组降低。与其他组相比，VCD 组雌二醇水平降低。与 VCD 组相比，VCD+ALA 组 MDA 值显著升高。此外，与 VCD 组相比，VCD+ALA 组 GSH 值降低。
结论
ALA 治疗 VCD 诱导的 POF 大鼠对减少卵巢损伤具有有益作用，可改善组织学、免疫组织化学、激素水平和氧化应激标志物。我们的结果表明，ALA 是治疗 VCD 诱导的 POF 大鼠的有效方法。
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