Abolaji Amos O, Omozokpia Marvis U, Oluwamuyide Olajide J, Akintola Temidayo E, Farombi Ebenezer O
Drug Metabolism and Molecular Toxicology Research Laboratories, Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Ibadan, Nigeria, Phone: +2348068614194.
Drug Metabolism and Molecular Toxicology Research Laboratories, Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Ibadan, Nigeria.
J Basic Clin Physiol Pharmacol. 2020 Mar 11;31(2):/j/jbcpp.2020.31.issue-2/jbcpp-2018-0115/jbcpp-2018-0115.xml. doi: 10.1515/jbcpp-2018-0115.
Background The ovotoxicity of 4-vinylcyclohexene diepoxide (VCD) has been established in several experimental models. Hesperidin (HSD) is a bi-flavonoid found in citrus fruits and has been reported to be a potent antioxidant and anti-inflammatory agent. Here, we have evaluated the rescue role of hesperidin on VCD-induced toxicity in the brain, ovary, and uterus of rats. Methods Six groups of rats containing ten rats in each group were orally given corn oil (control), hesperidin (100 mg/kg), hesperidin (200 mg/kg), VCD (250 mg/kg), VCD [(250 mg/kg)+hesperidin (100 mg/kg)] and VCD [(250 mg/kg)+hesperidin (200 mg/kg)] once a day for 30 days, respectively. Thereafter, we determined the selected biomarkers of oxidative damage, inflammation, endocrine balance, and histology of the reproductive organs. Results The data showed that hesperidin rescued VCD-induced increase in oxidative stress (hydrogen peroxide and malondialdehyde) and inflammatory (nitric oxide) biomarkers. In addition, hesperidin restored the reduction in antioxidant enzymes (catalase, glutathione S-transferase, glutathione peroxidase) activities and glutathione level in the brain, ovary, and uterus of rats (p<0.05). Lastly, hesperidin preserved the histological structure of the ovary and uterus of rats exposed to VCD. Conclusions Overall, the rescue role of hesperidin on VCD-induced toxicity in the brain and reproductive organs of female rats may be due to its antioxidative and anti-inflammatory properties.
4-乙烯基环己烯二环氧化物（VCD）的卵巢毒性已在多个实验模型中得到证实。橙皮苷（HSD）是一种存在于柑橘类水果中的双黄酮，据报道是一种有效的抗氧化剂和抗炎剂。在此，我们评估了橙皮苷对VCD诱导的大鼠脑、卵巢和子宫毒性的挽救作用。
将六组大鼠，每组十只，分别口服玉米油（对照组）、橙皮苷（100mg/kg）、橙皮苷（200mg/kg）、VCD（250mg/kg）、VCD[（250mg/kg）+橙皮苷（100mg/kg）]和VCD[（250mg/kg）+橙皮苷（200mg/kg）]，每天一次，持续30天。此后，我们测定了氧化损伤、炎症、内分泌平衡的选定生物标志物以及生殖器官的组织学。
数据显示，橙皮苷挽救了VCD诱导的氧化应激（过氧化氢和丙二醛）和炎症（一氧化氮）生物标志物的增加。此外，橙皮苷恢复了大鼠脑、卵巢和子宫中抗氧化酶（过氧化氢酶、谷胱甘肽S-转移酶、谷胱甘肽过氧化物酶）活性和谷胱甘肽水平的降低（p<0.05）。最后，橙皮苷保留了暴露于VCD的大鼠卵巢和子宫的组织结构。
总体而言，橙皮苷对VCD诱导的雌性大鼠脑和生殖器官毒性的挽救作用可能归因于其抗氧化和抗炎特性。
