Ndiaye Tolla, Sy Mouhamad, Gaye Amy, Ndiaye Daouda
Laboratory of Parasitology/Mycology HALD, Cheikh Anta Diop University of Dakar, PO Box 5005, Dakar, Senegal.
Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA, USA.
Afr Health Sci. 2019 Sep;19(3):2446-2456. doi: 10.4314/ahs.v19i3.19.
Despite a significant decline in Senegal, malaria remains a burden in various parts of the country. Assessment of multiplicity of infection and genetic diversity of parasites population could help in monitoring of malaria control.
To assess genetic diversity and multiplicity of infection in isolates from three areas in Senegal with different malaria transmissions.
136 blood samples were collected from patients with uncomplicated malaria in Pikine, Kedougou and Thies. Polymorphic loci of msp1 and 2 (Merozoite surface protein-1 and 2) genes were amplified by nested PCR.
For msp1gene, K1 allelic family was predominant with frequency of 71%. Concerning msp2 gene, IC3D7 allelic family was the most represented with frequency of 83%. Multiclonal isolates found were 36% and 31% for msp1et msp2 genes respectively. The MOI found in all areas was 2.56 and was statistically different between areas (P=0.024). Low to intermediate genetic diversity were found with heterozygosity range (He=0,394-0,637) and low genetic differentiation (Fst msp1= 0.011; Fst msp2=0.017) were observed between population within the country.
Low to moderate genetic diversity of strains and MOI disparities were found in Senegal.
尽管塞内加尔的疟疾发病率显著下降，但该国不同地区仍面临疟疾负担。评估寄生虫群体的感染多样性和基因多样性有助于监测疟疾控制情况。
评估塞内加尔三个疟疾传播情况不同地区分离株的基因多样性和感染多样性。
从皮基内、凯杜古和捷斯的非重症疟疾患者中采集了136份血样。通过巢式PCR扩增msp1和2（裂殖子表面蛋白-1和2）基因的多态性位点。
对于msp1基因，K1等位基因家族占主导地位，频率为71%。关于msp2基因，IC3D7等位基因家族占比最高，频率为83%。msp1和msp2基因的多克隆分离株分别为36%和31%。所有地区的感染复数为2.56，且各地区之间存在统计学差异（P = 0.024）。发现遗传多样性低至中等，杂合度范围为（He = 0.394 - 0.637），国内群体之间观察到低水平的遗传分化（Fst msp1 = 0.011；Fst msp2 = 0.017）。
塞内加尔发现菌株的遗传多样性低至中等，且感染复数存在差异。
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