Sir William Dunn School of Pathology, University of Oxford, Oxford, UK.
Wiley Interdiscip Rev RNA. 2020 Sep;11(5):e1593. doi: 10.1002/wrna.1593. Epub 2020 Mar 3.
RNA synthesis by RNA polymerase II and RNA processing are closely coupled during the transcription cycle of protein-coding genes. This coupling affords opportunities for quality control and regulation of gene expression and the effects can go in both directions. For example, polymerase speed can affect splice site selection and splicing can increase transcription and affect the chromatin landscape. Here we review the many ways that transcription and splicing influence one another, including how splicing "talks back" to transcription. We will also place the connections between transcription and splicing in the context of other RNA processing events that define the exons that will make up the final mRNA. This article is categorized under: RNA Processing > Splicing Mechanisms RNA Processing > Splicing Regulation/Alternative Splicing.
RNA 聚合酶 II 介导的 RNA 合成和 RNA 加工在蛋白编码基因的转录循环中紧密偶联。这种偶联为基因表达的质量控制和调控提供了机会，其影响可以是双向的。例如，聚合酶的速度可以影响剪接位点的选择，而剪接可以增加转录并影响染色质景观。在这里，我们回顾了转录和剪接相互影响的许多方式，包括剪接如何“反向”影响转录。我们还将把转录和剪接之间的联系放在其他 RNA 加工事件的背景下，这些事件定义了构成最终 mRNA 的外显子。本文属于以下分类：RNA 加工 > 剪接机制 RNA 加工 > 剪接调控/可变剪接。
