Department of Pulmonary Medicine, Faculty of Medicine, Inonu University, Malatya, Turkey.
Department of Pharmacology, Faculty of Medicine, Inonu University, Malatya, Turkey.
J Bras Pneumol. 2020 Mar 2;46(2):e20180406. doi: 10.36416/1806-3756/e20180406. eCollection 2020.
This study aimed to investigate acute and chronic effects of varenicline on lung tissue in an experimental study.
A total of 34 rats were randomly allocated into study (varenicline) and control groups. The rats were divided into two groups (i) control group, (ii) varenicline group. Then, the rats in the each group were sub-divided equally in turn as acute (C1; V1) and chronic (C2; V2) ; all rats of acute and chronic groups were sacrificed under the anesthesia on the 45th day for acute group [C1 (n=5) and V1 (n=12)] and the 90th day for chronic group [C2 (n=5) and V2 (n=12)], respectively. Thus, biochemical and histopathological analysis were carried out.
Thirty four rats completed the study, 24 were in varenicline group and 10 were in control group. In chronic exposure to varenicline, oxidant levels comprising of malondialdehyde (MDA), and myeloperoxidase (MPO) increased and superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) and glutathione peroxidase (GPx) levels, named as antioxidants, decreased significantly when compared to the control group. MDA and MPO levels were also significantly higher and SOD, CAT, GPx, GSH levels were also significantly lower in chronic varenicline group when compared to acute varenicline group. These findings were also supported by histopathological observations.
This is the first study, which evaluated pulmonary effects of varenicline experimentally on an animal model. It was observed that chronic varenicline treatments cause inflammation and lung cell injury.
本研究旨在通过实验研究探讨伐尼克兰对肺组织的急性和慢性影响。
共 34 只大鼠随机分为研究（伐尼克兰）和对照组。大鼠分为两组：（i）对照组，（ii）伐尼克兰组。然后，每组大鼠进一步分为急性组（C1；V1）和慢性组（C2；V2）；急性组的所有大鼠（C1[ n=5]和 V1[ n=12]）和慢性组的所有大鼠（C2[ n=5]和 V2[ n=12]）在第 45 天和第 90 天分别在麻醉下处死，进行生化和组织病理学分析。
34 只大鼠完成了研究，其中 24 只在伐尼克兰组，10 只在对照组。在慢性暴露于伐尼克兰时，氧化应激标志物丙二醛（MDA）和髓过氧化物酶（MPO）的水平升高，而超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽（GSH）和谷胱甘肽过氧化物酶（GPx）等抗氧化剂的水平降低，与对照组相比有显著差异。与急性伐尼克兰组相比，慢性伐尼克兰组的 MDA 和 MPO 水平也显著升高，SOD、CAT、GPx、GSH 水平也显著降低。这些发现也得到了组织病理学观察的支持。
这是第一项在动物模型上评估伐尼克兰对肺部影响的研究。结果表明，慢性伐尼克兰治疗可引起炎症和肺细胞损伤。
