Institute of Medical Research, Northwestern Polytechnical University, 127 West Youyi Road, Xi'an, Shaanxi 710072, China.
Department of Ultrasonography, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China.
Clinics (Sao Paulo). 2020 Feb 27;75:e1339. doi: 10.6061/clinics/2020/e1339. eCollection 2020.
Cerebral ischemia seriously threatens human health and is characterized by high rates of incidence, disability and death. Developing an ideal animal model of cerebral ischemia that reflects the human clinical features is critical for pathological studies and clinical research. The goal of this study is to establish a local cerebral ischemia model in rhesus macaque, thereby providing an optimal animal model to study cerebral ischemia.
Eight healthy rhesus monkeys were selected for this study. CT scans were performed before the operation to exclude cerebral vascular and intracranial lesions. Under guidance and monitoring with digital subtraction angiography (DSA), a microcatheter was inserted into the M1 segment of the middle cerebral artery (MCA) via the femoral artery. Then, autologous white thrombi were introduced to block blood flow. Immediately following embolization, multisequence MRI was used to monitor cerebrovascular and brain parenchymal conditions. Twenty-four hours after embolization, 2 monkeys were sacrificed and subjected to perfusion, fixation and pathological examination.
The cerebral ischemia model was established in 7 rhesus monkeys; one animal died during intubation. DSA and magnetic resonance angiography (MRA) indicated the presence of an arterial occlusion. MRI showed acute local cerebral ischemia. HE staining revealed infarct lesions formed in the brain tissues, and thrombi were present in the cerebral artery.
We established a rhesus macaque model of local cerebral ischemia by autologous thrombus placement. This model has important implications for basic and clinical research on cerebral ischemia. MRI and DSA can evaluate the models to ensure accuracy and effectiveness.
脑缺血严重威胁人类健康，其发病率、致残率和死亡率均较高。开发一种理想的反映人类临床特征的脑缺血动物模型对于病理研究和临床研究至关重要。本研究旨在建立恒河猴局部脑缺血模型，从而为研究脑缺血提供一种最佳的动物模型。
本研究选取 8 只健康恒河猴，在术前进行 CT 扫描以排除脑血管和颅内病变。在数字减影血管造影（DSA）的引导和监测下，通过股动脉将微导管插入大脑中动脉（MCA）的 M1 段。然后，将自体白血栓引入以阻断血流。栓塞后立即进行多序列 MRI 监测脑血管和脑实质情况。栓塞后 24 小时，2 只猴子被处死并进行灌注、固定和病理检查。
7 只恒河猴成功建立了脑缺血模型，1 只动物在插管过程中死亡。DSA 和磁共振血管造影（MRA）显示动脉闭塞。MRI 显示急性局部脑缺血。HE 染色显示脑组织中形成梗死病灶，脑动脉内有血栓。
我们通过自体血栓放置建立了恒河猴局部脑缺血模型。该模型对于脑缺血的基础和临床研究具有重要意义。MRI 和 DSA 可用于评估模型，以确保其准确性和有效性。
