Biology Department (Departamento de Biología), Universidad del Valle, Street 13 No. 100-00, Cali, 760032, Colombia.
Curr Microbiol. 2020 Jul;77(7):1283-1291. doi: 10.1007/s00284-020-01939-y. Epub 2020 Mar 4.
Insects have established close relationships with a wide variety of microorganisms, which play a key role in insect ecology and evolution. Fruit flies in the Tephritidae family have economic importance at the global level, including species such as Anastrepha obliqua, which is an important pest in the neotropical region. Although several studies have been performed on the microbiota associated with fruit flies, there are still large gaps in our knowledge about the bacterial communities on the genus Anastrepha. During this study, we used high-throughput sequencing to characterize the bacterial communities of the polyphagous fly A. obliqua, and we evaluated the effect of the life stage (larvae and adults) and host plant (three plant species) on the structure of these communities. Our results show that the bacterial communities in A. obliqua appears to be structured according to the insect life stage and the host plant. The predominant genera belonging to the phylum Proteobacteria were Wolbachia and Enterobacter in both larvae and adults, and they displayed differences in abundance between them, with Wolbachia sp. being more abundant in larvae and Enterobacter sp. being more abundant in adults. Differences in the structures of the bacterial communities were also observed according to the host plant with higher abundance of Enterobacter and Acetobacter bacteria in mango and plum fruits. Based on our results, it can be hypothesized that the bacterial communities on A. obliqua reorganize according to the needs of these insects during their different life stages and could also play an important role in the establishment of this fly species on different host plants. This study represents the first approach to understanding microorganism-insect interactions in fruit flies in Colombia.
昆虫与各种各样的微生物建立了密切的关系，这些微生物在昆虫的生态和进化中起着关键作用。在全球范围内，实蝇科的果蝇具有经济重要性，包括安氏亚种等在新热带地区重要的害虫。尽管已经对与果蝇相关的微生物群进行了多项研究，但我们对安氏属果蝇的细菌群落仍知之甚少。在这项研究中，我们使用高通量测序来描述多食性果蝇 A. obliqua 的细菌群落，并评估了生活阶段（幼虫和成虫）和宿主植物（三种植物物种）对这些群落结构的影响。我们的研究结果表明，A. obliqua 的细菌群落似乎根据昆虫的生活阶段和宿主植物而有所结构。主要属于变形菌门的属细菌是幼虫和成虫中的沃尔巴克氏体和肠杆菌属，它们的丰度存在差异，其中沃尔巴克氏体在幼虫中更为丰富，肠杆菌属在成虫中更为丰富。根据宿主植物的不同，细菌群落的结构也存在差异，在芒果和李子果实中，肠杆菌属和醋杆菌属的细菌丰度更高。根据我们的研究结果，可以假设 A. obliqua 上的细菌群落根据这些昆虫在不同生活阶段的需求进行重组，并且可能在该果蝇种在不同宿主植物上的建立中发挥重要作用。本研究代表了首次尝试了解哥伦比亚果蝇中微生物-昆虫相互作用的研究。
