Laboratorio de Biotecnología Ambiental y Agroecológica, Grupo Académico de Biotecnología Ambiental, El Colegio de La Frontera Sur (ECOSUR), Tapachula, Chiapas, Mexico.
Programa Moscafrut DGSV-SENASICA-SAGARPA, Subdirección de Desarrollo de Métodos, Chiapas, Mexico.
Arch Microbiol. 2022 Aug 27;204(9):579. doi: 10.1007/s00203-022-03207-y.
The fruit fly Anastrepha obliqua is an economically important pest. The sterile insect technique to control it involves mass production and release of sterile flies to reduce the reproduction of the wild population. As noted in different Tephritidae, the performance of sterile males may be affected by the assimilation of nutrients under mass-rearing conditions. In the wild, the fly's life cycle suggests the acquisition of different organisms that could modulate its fitness and physiology. For A. obliqua, there is no information regarding microorganisms other than bacteria. This study analyzed bacteria, fungal, and archaea communities in the A. obliqua gut through denaturing gradient gel electrophoresis (DGGE) profiles of 16S (using a different set of primers for bacteria and archaea) and 18S ribosomal DNA markers. We found that wild flies presented higher microbial diversity related to fructose assimilation than laboratory species, suggesting that microorganisms have led to a specialized metabolism to process nutrients associated with an artificial diet. We identified species that have not been previously described in this fruit fly, especially actinobacteria and archaea, by employing different primer sets aimed at the same molecular marker but targeting diverse hypervariable regions of 16S rDNA. The possibility that Archaea affect fly fitness should not be ignored. This report on the intestinal microbial (bacteria, archaea, and fungi) composition of A. obliqua contributes to our understanding of the role of microorganisms in the development and physiology of the flies.
果实蝇 Anastrepha obliqua 是一种具有重要经济意义的害虫。利用不育昆虫技术来控制它，涉及到大量生产和释放不育蝇，以减少野生种群的繁殖。正如在不同的实蝇科中所指出的，不育雄蝇的性能可能会受到大规模饲养条件下营养物质同化的影响。在野外，蝇的生命周期表明它会获取不同的生物体，这些生物体可能会调节它的适应性和生理学。对于 A. obliqua，除了细菌之外，没有关于其他微生物的信息。本研究通过变性梯度凝胶电泳 (DGGE) 分析了 A. obliqua 肠道中的细菌、真菌和古菌群落，使用了针对细菌和古菌的不同 16S 引物（18S 核糖体 DNA 标记物）。我们发现，野生蝇比实验室蝇具有更高的与果糖同化相关的微生物多样性，这表明微生物导致了一种专门的代谢途径，以处理与人工饮食相关的营养物质。我们通过使用不同的引物对同一分子标记物，但针对 16S rDNA 的不同高变区，鉴定出了以前在这种果实蝇中未被描述过的物种，特别是放线菌和古菌。古菌可能会影响蝇的适应性，这一点不容忽视。本报告关于 A. obliqua 肠道微生物（细菌、古菌和真菌）组成的研究有助于我们理解微生物在蝇的发育和生理学中的作用。
