Onele A O, Chasov A V, Trifonova T V, Minibayeva F V
Kazan (Volga Region) Federal University, Kazan, Russian Federation.
Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Center, Russian Academy of Sciences, Kazan, Russian Federation.
Dokl Biochem Biophys. 2019 Nov;489(1):380-384. doi: 10.1134/S1607672919060085. Epub 2020 Mar 4.
In the present work, the APX gene encoding ascorbate peroxidase in the moss Dicranum scoparium was for the first time cloned and sequenced, and a high homology of APX with ascorbate peroxidase genes of the mosses Grimmia pilifera and Physcomitrella patens was shown. The structure of the protein was characterized using bioinfomatics approach, and the activity of the enzyme under abiotic stresses was studied. An increase in the activity of ascorbate peroxidase was detected during desiccation of D. scoparium shoots. When exposed to heat shock, a decrease in the activity of ascorbate peroxidase correlated with a decrease in the expression of APX. Conserved elements, which were found in the structure of ascorbate peroxidase gene and protein, indicate that these sequences are conserved in the plant genome during evolution, in support of the importance of this enzyme in maintaining cellular redox status.
在本研究中，首次克隆并测序了苔藓曲尾藓中编码抗坏血酸过氧化物酶的APX基因，结果表明该基因与藓类植物丛毛藓和小立碗藓的抗坏血酸过氧化物酶基因具有高度同源性。采用生物信息学方法对该蛋白的结构进行了表征，并研究了非生物胁迫下该酶的活性。在曲尾藓茎干燥过程中检测到抗坏血酸过氧化物酶活性增加。当受到热激时，抗坏血酸过氧化物酶活性降低，且与APX基因表达下降相关。在抗坏血酸过氧化物酶基因和蛋白结构中发现的保守元件表明，这些序列在植物基因组进化过程中是保守的，这支持了该酶在维持细胞氧化还原状态中的重要性。
