Galam Nanyak, Tulay Pinar, Adali Terin
Tissue Engineering and Biomaterials Research Center, Near East University, P.O. Box 99138 North Cyprus, 10 99138 Mersin, Turkey.
Department of Biomedical Engineering, Faculty of Engineering, Near East University, P.O. Box 99138 North Cyprus, 10 99138 Mersin, Turkey.
Molecules. 2020 Mar 2;25(5):1110. doi: 10.3390/molecules25051110.
Breast cancer ranks as the fifth leading cause of death worldwide. Chemotherapy is commonly used directly or as neo-adjuvant therapy for the management of breast cancer with its attendant adverse effects, underscoring the need to develop biocompatible bioactive compounds for pharmacological applications. The aim of this study is to encapsulate carboplatin (CP) with silk fibroin protein (SF) by using an ionic gelation method as a drug carrier system and assess the apoptotic effect on MCF-7 breast cancer cells during in vitro studies. The characterization of silk fibroin encapsulated carboplatin (SFCP) microparticles was analyzed by FTIR spectrophotometer, SEM, Mastersizer, and biodegradation methods. The encapsulation efficiency and release profile of SFCP microparticles were analyzed by an indirect UV-Vis spectrophotometric method. An apoptotic screening of MCF-7 cells was carried out with 10-200 µg/mL CP loaded SFCP, which were cultured for 24, 48, and 72 h. Data were analyzed using the Student's test and analysis of variance. FTIR and drug release studies confirmed an interaction of silk fibroin with the carboplatin moiety. SFCP showed successful encapsulation of the carboplatin moiety. Apoptotic screening showed a dose dependent increase in absorbance, indicating significant cell death ( < 0.05). Thus, the direct apoptotic effect of SFCP microparticles on MCF-7 was confirmed.
乳腺癌是全球第五大死因。化疗是治疗乳腺癌常用的直接治疗手段或新辅助治疗方法，但会产生相应的副作用，这凸显了开发用于药理学应用的生物相容性生物活性化合物的必要性。本研究的目的是通过离子凝胶法将卡铂（CP）与丝素蛋白（SF）包裹在一起，作为一种药物载体系统，并在体外研究中评估其对MCF-7乳腺癌细胞的凋亡作用。通过傅里叶变换红外光谱仪、扫描电子显微镜、激光粒度分析仪和生物降解方法对丝素蛋白包裹卡铂（SFCP）微粒进行表征分析。采用间接紫外可见分光光度法分析SFCP微粒的包封率和释放曲线。用负载10 - 200μg/mL CP的SFCP对MCF-7细胞进行凋亡筛选，细胞培养24、48和72小时。数据采用学生t检验和方差分析进行分析。傅里叶变换红外光谱和药物释放研究证实了丝素蛋白与卡铂部分之间的相互作用。SFCP成功包裹了卡铂部分。凋亡筛选显示吸光度呈剂量依赖性增加，表明细胞死亡显著（P<0.05）。因此，证实了SFCP微粒对MCF-7细胞有直接凋亡作用。
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