Khan Md Asad, Zafaryab Md, Mehdi Syed Hassan, Quadri Javed, Rizvi M Moshahid A
Genome Biology Lab, Department of Biosciences, Jamia Millia Islamia, New Delhi, 110025, India.
Genome Biology Lab, Department of Biosciences, Jamia Millia Islamia, New Delhi, 110025, India.
Int J Biol Macromol. 2017 Apr;97:115-122. doi: 10.1016/j.ijbiomac.2016.12.090. Epub 2017 Jan 9.
Aim of the studies to synthesized chitosan nanoparticles by an ionic interaction procedure. The nanoparticles were characterized by physicochemical methods like, DLS, TEM, Surface potential measurements, FT-IR and DSC. The average particle size of chitosan and carboplatin nanoparticles was found to be 277.25±11.37nm and 289.30±8.15nm and zeta potential was found to be 31±3.14mV and 33±2.15mV respectively with low polydispersity index. The maximum entrapment of carboplatin in nanoparticles was a spherical shape with a positive charge. The maximum encapsulation and loading efficiencies of carboplatin (5mg/ml) were obtained to be 58.43% and 13.27% respectively. The nanocarboplatin was better blood compatibility as compared to chitosan nanoparticles. Finally, the cytotoxic effects of the carboplatin loaded chitosan nanoparticles were tested in-vitro against breast cancer (MCF-7) cell lines. Our studies showed that the chitosan nanoparticles could be used as a promising candidate for drug delivery for the therapeutic treatment of breast cancer.
这些研究的目的是通过离子相互作用法合成壳聚糖纳米颗粒。采用动态光散射（DLS）、透射电子显微镜（TEM）、表面电位测量、傅里叶变换红外光谱（FT-IR）和差示扫描量热法（DSC）等物理化学方法对纳米颗粒进行表征。壳聚糖和卡铂纳米颗粒的平均粒径分别为277.25±11.37nm和289.30±8.15nm，zeta电位分别为31±3.14mV和33±2.15mV，多分散指数较低。纳米颗粒中卡铂的最大包封率为球形且带正电荷。卡铂（5mg/ml）的最大包封率和载药效率分别为58.43%和13.27%。与壳聚糖纳米颗粒相比，纳米卡铂具有更好的血液相容性。最后，对负载卡铂的壳聚糖纳米颗粒对乳腺癌（MCF-7）细胞系进行体外细胞毒性测试。我们 的研究表明，壳聚糖纳米颗粒有望成为用于乳腺癌治疗的药物递送候选物。
