Wieser H, Seilmeier W, Belitz H D
Deutsche Forschungsanstalt für Lebensmittelchemie, Institut für Lebensmittelchemie der Technischen Universität München, Garching, Federal Republic of Germany.
Z Lebensm Unters Forsch. 1988 Jul;187(1):27-34. doi: 10.1007/BF01454319.
The peptide fractions isolated from chymotryptic hydrolysates of wheat, rye and barley glutelins were separated by high-performance liquid chromatography on octadecyl silica gel. The peptides obtained were analysed for their amino acid composition and some also for their amino acid sequence. Characteristic sequences of peptides from wheat glutelin can be grouped into three types. The first type contains a high amount of Gly and frequently Tyr in the N-terminal positions. A typical partial sequence, which occurs repeated in two peptides, is QGQQPGQGQ. Sequences of this type are found in high-molecular-weight subunits. The second type is characterized by the sequence SQn (n = 3-5) followed by a hydrophobic tripeptide e.g., PPF, PVL; the most frequent sequence is SQQQQPPF. Low-molecular-weight subunits contain sequences of this type. The third type, which has partial sequences such as QQPQQPFP, corresponds to typical peptides from prolamin. Most sequences of peptides from rye and barley glutelins can be divided into two groups. The predominant type shows prolamin-like sequences, e.g., PQQPXPQQ with X being F or I. The second type is similar to glycine-rich peptides from wheat glutelin, except that repeating sequences are less frequent.
从小麦、黑麦和大麦谷蛋白的胰凝乳蛋白酶水解产物中分离得到的肽段，通过十八烷基硅胶上的高效液相色谱进行分离。对所得肽段进行氨基酸组成分析，部分肽段还进行了氨基酸序列分析。小麦谷蛋白肽段的特征序列可分为三种类型。第一种类型在N端位置含有大量的甘氨酸，并且经常含有酪氨酸。在两个肽段中重复出现的一个典型部分序列是QGQQPGQGQ。这种类型的序列存在于高分子量亚基中。第二种类型的特征是序列SQn（n = 3 - 5），后面跟着一个疏水性三肽，例如PPF、PVL；最常见的序列是SQQQQPPF。低分子量亚基含有这种类型的序列。第三种类型具有诸如QQPQQPFP等部分序列，对应于醇溶蛋白的典型肽段。黑麦和大麦谷蛋白肽段的大多数序列可分为两组。主要类型显示出醇溶蛋白样序列，例如PQQPXPQQ，其中X为F或I。第二种类型类似于来自小麦谷蛋白的富含甘氨酸的肽段，只是重复序列较少。
