喹诺酮耐药性、基因与整合子携带情况在多药耐药社区分离的 spp. 菌株中的相关性
Correlation of quinolone-resistance, genes and integron carriage in multidrug-resistant community isolates of spp.
作者信息
Malek Jamshidi Mohammad Reza, Zandi Hengameh, Eftekhar Fereshteh
机构信息
Department of Microbiology, Faculty of Life Sciences and Technology, Shahid Beheshti University, Tehran, Iran.
Department of Microbiology, Research Center for Food Hygiene and Safety, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
出版信息
Iran J Basic Med Sci. 2019 Dec;22(12):1387-1391. doi: 10.22038/IJBMS.2019.13985.
OBJECTIVES
Plasmid-mediated quinolone resistance (PMQR) determinants and integrons have a considerable contribution to bacterial drug resistance in Gram-negative pathogens. We studied the prevalence of PMQR genes and integron carriage in multidrug-resistant community isolates of spp.
MATERIALS AND METHODS
Two hundred and fifty spp. isolates were collected from outpatient specimens between August 2015 and October 2017 in Yazd central Laboratory, Iran. Antibiotic susceptibility was determined against 17 antibiotics and minimum inhibitory concentration (MIC) of ciprofloxacin was measured by E-test. Polymerase chain reaction (PCR) was employed for detection of and genes.
RESULTS
Disc diffusion results showed that 17 isolates (6.8%) were multidrug resistant (MDR), two of which were and 15 were . MIC measurements revealed 11 ciprofloxacin-resistant isolates (including the two ), three intermediately-resistant and three ciprofloxacin-susceptible isolates. All ciprofloxacin-resistant and intermediately-resistant isolates carried at least one and up to four PMQR genes. The most prevalent PMQR gene was oqxAB (93.75%) followed by aac(6')-ib-cr (50.0%), (25.0%) and (18.75%) but and were not detected. Class 1 integron was observed in 14 (82.3%) isolates including nine ciprofloxacin-resistant, two intermediately-resistant, and three susceptible isolates. Class 2 and 3 integrons were not observed.
CONCLUSION
Presence of MDR, multiple PMQR determinants as well as class 1 integron in community isolates of spp. can be an important source of transmission of these opportunistic pathogens.
目的
质粒介导的喹诺酮耐药性（PMQR）决定簇和整合子对革兰氏阴性病原体的细菌耐药性有相当大的影响。我们研究了PMQR基因的流行情况以及在多药耐药社区分离株中的整合子携带情况。
材料与方法
2015年8月至2017年10月期间，从伊朗亚兹德中央实验室的门诊标本中收集了250株分离株。测定了对17种抗生素的药敏性，并通过E试验测量了环丙沙星的最低抑菌浓度（MIC）。采用聚合酶链反应（PCR）检测和基因。
结果
纸片扩散法结果显示，17株分离株（6.8%）为多药耐药（MDR），其中2株为，15株为。MIC测定显示有11株环丙沙星耐药分离株（包括2株）、3株中介耐药和3株环丙沙星敏感分离株。所有环丙沙星耐药和中介耐药分离株携带至少1个至4个PMQR基因。最常见的PMQR基因是oqxAB（93.75%），其次是aac(6')-ib-cr（50.0%）、（25.0%）和（18.75%），但未检测到和。在14株（82.3%）分离株中观察到1类整合子，包括9株环丙沙星耐药、2株中介耐药和3株敏感分离株。未观察到2类和3类整合子。
结论
在分离株的社区分离株中存在多药耐药、多种PMQR决定簇以及1类整合子可能是这些机会性病原体传播的重要来源。
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