Amereh Fereshteh, Arabestani Mohammad Reza, Hosseini Seyed Mostafa, Shokoohizadeh Leili
Student Research Committee, Hamadan University of Medical Sciences, Hamadan, Iran.
Infectious Disease Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
Int J Microbiol. 2023 Feb 21;2023:9199108. doi: 10.1155/2023/9199108. eCollection 2023.
The aim of this study was to investigate the frequency and relationship between plasmid-mediated quinolone resistance genes and OqxAB pump genes, as well as the genetic linkage in strains isolated from Hamadan hospitals in the west of Iran.
In this study, 100 clinical strains were isolated from clinical samples of inpatients at Hamadan Hospital in 2021. The antimicrobial susceptibility testing was performed using the disk diffusion method. The frequencies of genes encoding OqxAB efflux pumps and were investigated by PCR. Molecular typing of -positive isolates was assessed by ERIC-PCR.
Antibiotic susceptibility testing showed high resistance (>80%) to fluoroquinolones. The gene encoding the OqxAB efflux pump was detected in more than 90% of strains. All isolates were negative for A, and 20% and 9% of the isolates were positive for B and S, respectively. The genes encoding A and B were detected in 96% of -positive strains. A B + /S + profile was observed in 16% of -positive strains. Ciprofloxacin MIC ≥ 256 g/ml was detected in 20% of -positive strains. Genetic association analysis by ERIC-PCR revealed genetic diversity among 25 different -positive strains of .
However, no significant correlation was found between the and the OqxAB efflux pump genes in this study. The high rate of fluoroquinolone resistance and determinants of antibiotic resistance among diverse strains increase the risk of fluoroquinolone-resistance transmission by strains in hospitals.
本研究旨在调查伊朗西部哈马丹医院分离菌株中质粒介导的喹诺酮耐药基因与OqxAB泵基因之间的频率及关系，以及基因连锁情况。
本研究于2021年从哈马丹医院住院患者的临床样本中分离出100株临床菌株。采用纸片扩散法进行抗菌药物敏感性试验。通过PCR研究编码OqxAB外排泵的基因频率。通过ERIC-PCR评估阳性分离株的分子分型。
抗生素敏感性试验显示对氟喹诺酮类药物的耐药率较高（>80%）。在90%以上的菌株中检测到编码OqxAB外排泵的基因。所有分离株A均为阴性，20%和9%的分离株B和S分别为阳性。在96%的阳性菌株中检测到编码A和B的基因。在16%的阳性菌株中观察到B + /S + 谱型。在20%的阳性菌株中检测到环丙沙星MIC≥256μg/ml。通过ERIC-PCR进行的遗传关联分析揭示了25种不同阳性菌株之间的遗传多样性。
然而，本研究中未发现与OqxAB外排泵基因之间存在显著相关性。不同菌株中氟喹诺酮耐药率高以及抗生素耐药决定因素增加了医院菌株传播氟喹诺酮耐药性的风险。
