PMQR 基因在摩洛哥西北部分离的大肠杆菌和克雷伯氏菌属中的流行情况。
Prevalence of PMQR genes in E. coli and Klebsiella spp. isolated from North-West of Morocco.
机构信息
Lab. Valorisation Biotechnologique des Microorganismes, Génomique et Bioinformatique, Faculty of Sciences and Techniques, University Abdelmalek Essaadi, Tangier, Morocco; Laboratory of Microbiology, Research Department, Institut Pasteur du Maroc, Tangier 90000, Morocco.
Lab. Valorisation Biotechnologique des Microorganismes, Génomique et Bioinformatique, Faculty of Sciences and Techniques, University Abdelmalek Essaadi, Tangier, Morocco.
出版信息
J Glob Antimicrob Resist. 2017 Sep;10:321-325. doi: 10.1016/j.jgar.2017.05.024. Epub 2017 Jul 19.
OBJECTIVES
Plasmid mediated quinolone resistance (PMQR) genes are increasingly detected worldwide. However, in Morocco a few studies have been carried out. The aim of this study was to investigate the prevalence of these determinants among Escherichia coli and Klebsiella spp. isolated from North-West of Morocco.
METHODS
The prevalence of PMQR genes was investigated among 398 E. coli and 118 Klebsiella spp. clinical isolates collected between 2012 and 2015 from North-West of Morocco. Antimicrobial susceptibility was determined by disc diffusion assay and minimal inhibitory concentrations (MIC) of quinolone were determined by micro-dilution. The screening of qnrA, qnrB, qnrC qnrS, Aac(6')-Ib and qepA genes were done by PCR, DNA sequencing and RFLP analyses.
RESULTS
Among 398 E. coli and 118 Klebsiella spp. analyzed, 51% of E. coli and 61% of Klebsiella spp. were multidrug resistant (MDR). For the E. coli group, qnrB, qnrS, and aac(6')-Ib-cr genes were detected in 05.02%, 01.50%, and 13.81%, respectively. These determinants were more prevalent in Klebsiella spp. group, hence they represented 11.86%, 05.93% and 18.64% respectively. QnrA, qnrC and qepA were absent in this study. For aac(6')Ib determinant, 84.41% were belong to the Aac(6') Ib-cr variant while for qnrB and qnrS the most determined variants were qnrB1, B6, B16, B42, B66 and qnrS1, S4, S7. Different plasmid sizes were detected in PMQR strains.
CONCLUSION
This is the first study conducted in North-West of Morocco and shows important dissemination of MDR and PMQR among Enterobacteriaceae.
目的
质粒介导的喹诺酮类耐药（PMQR）基因在世界范围内越来越多地被检测到。然而，在摩洛哥，仅有少数研究开展。本研究旨在调查这些决定因素在摩洛哥西北部分离的大肠杆菌和克雷伯氏菌中的流行情况。
方法
在 2012 年至 2015 年期间，从摩洛哥西北部收集了 398 株大肠杆菌和 118 株克雷伯氏菌临床分离株，调查了 PMQR 基因的流行情况。通过纸片扩散法测定抗菌药物敏感性，通过微量稀释法测定喹诺酮类药物的最小抑菌浓度（MIC）。通过 PCR、DNA 测序和 RFLP 分析筛查 qnrA、qnrB、qnrC、qnrS、Aac(6')-Ib 和 qepA 基因。
结果
在所分析的 398 株大肠杆菌和 118 株克雷伯氏菌中，51%的大肠杆菌和 61%的克雷伯氏菌为多重耐药（MDR）。对于大肠杆菌组，qnrB、qnrS 和 aac(6')-Ib-cr 基因的检出率分别为 05.02%、01.50%和 13.81%。这些决定因素在克雷伯氏菌组更为普遍，分别占 11.86%、05.93%和 18.64%。本研究中未发现 qnrA、qnrC 和 qepA。对于 aac(6')Ib 决定因素，84.41%属于 Aac(6') Ib-cr 变体，而对于 qnrB 和 qnrS，最确定的变体是 qnrB1、B6、B16、B42、B66 和 qnrS1、S4、S7。在 PMQR 菌株中检测到不同的质粒大小。
结论
这是在摩洛哥西北部进行的首次研究，表明 MDR 和 PMQR 在肠杆菌科中广泛传播。
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