Ahangarpour Akram, Oroojan Ali Akbar, Khorsandi Layasadat, Kouchak Maryam, Badavi Mohammad
Department of Physiology, Faculty of Medicine, Diabetes Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Physiology, Faculty of Medicine, Cellular and Molecular Research Center, Student Research Committee of Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Iran J Basic Med Sci. 2019 Dec;22(12):1424-1431. doi: 10.22038/IJBMS.2019.13989.
The present study evaluates the protective effects of myricitrin and its solid lipid nanoparticle (SLN) on diabetic nephropathy (DN) induced by streptozotocin-nicotinamide (STZ-NA) in mice.
In this experimental study, 108 adult male NMRI mice were divided into 9 groups: control, vehicle, diabetes, diabetes + myricitrin 1, 3, and 10 mg/kg and, diabetes + SLN containing myricitrin 1, 3, and 10 mg/kg. After the experimental period, the plasma and tissue samples were collected for experimental, histopathological, real-time PCR and apoptosis assessments.
Total antioxidant capacity, catalase, glomerular filtration rate, plasma level of albumin, urine (BUN) and, creatinine (Cr) levels decreased, and the kidney weight, intake/output, malondialdehyde, plasma level of BUN and Cr, urine level of sodium, potassium, albumin and glucose, fractional excretions of sodium and potassium, transforming growth factor-β (TGF-β) and nuclear factor kappa B (NF-κB) gene expression, red blood cell accumulation and infiltration of inflammatory cells, and kidney apoptosis increased in untreated diabetic mice compared to the control group, and administration of myricitrin and its SLN recovered all of these changes.
Ultimately, myricitrin and its SLN administration improved DN changes by reducing oxidative stress and increasing antioxidant enzymes level, and these effects were more prominent in the SLN-administered mice.
本研究评估杨梅素及其固体脂质纳米粒（SLN）对链脲佐菌素 - 烟酰胺（STZ - NA）诱导的小鼠糖尿病肾病（DN）的保护作用。
在本实验研究中，108只成年雄性NMRI小鼠分为9组：对照组、赋形剂组、糖尿病组、糖尿病 + 1、3和10 mg/kg杨梅素组以及糖尿病 + 含1、3和10 mg/kg杨梅素的SLN组。实验期结束后，收集血浆和组织样本进行实验、组织病理学、实时PCR和凋亡评估。
与对照组相比，未治疗的糖尿病小鼠的总抗氧化能力、过氧化氢酶、肾小球滤过率、血浆白蛋白水平、尿（尿素氮）和肌酐水平降低，而肾重量、摄入/输出、丙二醛、血浆尿素氮和肌酐水平、尿钠、钾、白蛋白和葡萄糖水平、钠和钾的分数排泄、转化生长因子 -β（TGF -β）和核因子κB（NF -κB）基因表达、红细胞聚集和炎性细胞浸润以及肾细胞凋亡增加，给予杨梅素及其SLN可恢复所有这些变化。
最终，给予杨梅素及其SLN可通过降低氧化应激和提高抗氧化酶水平改善DN变化，且这些作用在给予SLN的小鼠中更为显著。
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