Khazali Homayoun, Mahmoudi Fariba
Department of Animal Science and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.
Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, Iran.
Iran J Basic Med Sci. 2019 Dec;22(12):1462-1467. doi: 10.22038/IJBMS.2019.14053.
Kisspeptin and opioids are important factors for controlling GnRH/LH secretion. In present study, influences of kisspeptin or morphine were investigated on 5α- reductase or aromatase () genes expression in the hypothalamus, testis and liver. It aimed to investigate whether kisspeptin pathway may control morphine effects on plasma concentration of testosterone.
Twenty adult male rats in four groups received saline/saline, kisspeptin (1 nmol)/saline, morphine (5 mg/kg)/saline or kisspeptin/morphine respectively. Mean relative 5α-reductase and aromatase mRNA levels were determined by RT-PCR.
Morphine/saline injection increased significantly mean relative mRNA levels of hypothalamic 5α-reductase or aromatase compared to saline/saline. While morphine/saline did not alter mRNA levels of them compared to saline/saline group in the testis and liver. Kisspeptin/saline did not significantly decrease mean relative 5α-reductase or aromatase genes expression compared to saline/saline group in the hypothalamus, testis and liver. Injections of kisspeptin/morphine did not significantly decrease mean relative 5α-reductase or genes expression compared to morphine/saline group.
Up-regulation of hypothalamic 5α-reductase or aromatase mRNA levels may partly induce the inhibitory effects of morphine on GnRH/LH release. Different effects of morphine on aromatase or 5α- reductase genes expression levels in the liver and testis compared to brain may be partly due to different sensitivity or functions of them to morphine used dose.
亲吻素和阿片类物质是控制促性腺激素释放激素/促黄体生成素分泌的重要因素。在本研究中，研究了亲吻素或吗啡对下丘脑、睾丸和肝脏中5α-还原酶或芳香化酶（）基因表达的影响。旨在研究亲吻素途径是否可能控制吗啡对睾酮血浆浓度的影响。
20只成年雄性大鼠分为四组，分别接受生理盐水/生理盐水、亲吻素（1纳摩尔）/生理盐水、吗啡（5毫克/千克）/生理盐水或亲吻素/吗啡处理。通过逆转录聚合酶链反应测定平均相对5α-还原酶和芳香化酶mRNA水平。
与生理盐水/生理盐水组相比，注射吗啡/生理盐水显著提高了下丘脑5α-还原酶或芳香化酶的平均相对mRNA水平。而与生理盐水/生理盐水组相比，吗啡/生理盐水组对睾丸和肝脏中它们的mRNA水平没有影响。与下丘脑、睾丸和肝脏中的生理盐水/生理盐水组相比，亲吻素/生理盐水组并未显著降低平均相对5α-还原酶或芳香化酶基因表达。与吗啡/生理盐水组相比，注射亲吻素/吗啡并未显著降低平均相对5α-还原酶或基因表达。
下丘脑5α-还原酶或芳香化酶mRNA水平的上调可能部分诱导吗啡对促性腺激素释放激素/促黄体生成素释放的抑制作用。与大脑相比，吗啡对肝脏和睾丸中芳香化酶或5α-还原酶基因表达水平的不同影响可能部分归因于它们对所用吗啡剂量的不同敏感性或功能。
