Khazali Homayoun, Mahmoud Fariba
Department of Animal Science and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.
Department of Biology, Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, Iran.
Vet Res Forum. 2022 Mar;13(1):85-90. doi: 10.30466/vrf.2020.118362.2802. Epub 2022 Mar 15.
Kisspeptin is a hypothalamic peptide which stimulates hypothalamus- pituitary- gonadal (HPG) axis. Morphine is an alkaloid which suppresses reproduction. Ghrelin and leptin are metabolic peptides which play role in relaying information to the HPG axis. In the present study, the interaction effects of kisspeptin and morphine were investigated on plasma and gene expression levels of and . Twenty adult male Wistar rats randomized in four groups were received injection of saline, kisspeptin (1.00 nmol), morphine (5.00 mg kg) or Kisspeptin + morphine. Rats were received kisspeptin and morphine via third cerebral ventricular and subcutaneous injection, respectively. Ten male rats in two groups were received intravenous injection of saline or kisspeptin (7.50 nmol). Blood samples, hypothalamic and adipose tissue samples were collected. Plasma and gene expression levels of and were measured using the methods of enzyme-linked immunosorbent assay and real time-PCR, respectively. Morphine significantly increased plasma concentration and hypothalamic mRNA levels of compared to saline while kisspeptin significantly decreased them compared to saline. Morphine significantly decreased plasma and mRNA levels of in adipose tissue compared to saline, however, kisspeptin did not increase plasma and mRNA levels of in adipose tissue compared to saline. Kisspeptin significantly decreased the effects of morphine on plasma concentration and hypothalamic gene expression levels of compared to morphine alone, however, it did not affect morphine influence on plasma and gene expression levels compared to morphine alone. Kisspeptin and morphine might partly be involved in the regulation of reproductive activity via regulation the metabolic hormones synthesis.
亲吻素是一种刺激下丘脑 - 垂体 - 性腺（HPG）轴的下丘脑肽。吗啡是一种抑制生殖的生物碱。胃饥饿素和瘦素是在向HPG轴传递信息中起作用的代谢肽。在本研究中，研究了亲吻素和吗啡对[具体物质]的血浆和基因表达水平的相互作用。将20只成年雄性Wistar大鼠随机分为四组，分别接受生理盐水、亲吻素（1.00纳摩尔）、吗啡（5.00毫克/千克）或亲吻素 + 吗啡注射。大鼠分别通过第三脑室和皮下注射接受亲吻素和吗啡。两组中的10只雄性大鼠接受生理盐水或亲吻素（7.50纳摩尔）的静脉注射。采集血样、下丘脑和脂肪组织样本。分别使用酶联免疫吸附测定法和实时定量PCR法测量[具体物质]的血浆和基因表达水平。与生理盐水相比，吗啡显著提高了[具体物质]的血浆浓度和下丘脑mRNA水平，而亲吻素与生理盐水相比则显著降低了它们。与生理盐水相比，吗啡显著降低了脂肪组织中[具体物质]的血浆和mRNA水平，然而，亲吻素与生理盐水相比并未提高脂肪组织中[具体物质]的血浆和mRNA水平。与单独使用吗啡相比，亲吻素显著降低了吗啡对[具体物质]血浆浓度和下丘脑基因表达水平的影响，然而，与单独使用吗啡相比，它并未影响吗啡对血浆和[具体物质]基因表达水平的影响。亲吻素和吗啡可能部分通过调节代谢激素合成参与生殖活动的调节。
