Wu Xu, Dai Yang, Wang Liping, Peng Yan, Lu Lin, Zhu Yiming, Shi Yijue, Zhuang Songlin
Terahertz Technology Innovation Research Institute, Shanghai Key Lab of Modern Optical System, Terahertz Science Cooperative Innovation Center, University of Shanghai for Science and Technology, Shanghai, China.
Department of Cardiology, Rui Jin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China.
Biomed Opt Express. 2020 Jan 17;11(2):963-970. doi: 10.1364/BOE.381542. eCollection 2020 Feb 1.
Methyglyoxal (MGO) is an important pathological factor for diabetic cardiovascular complications. Conventional methods for MGO detection in biological samples, such as high performance liquid chromatography (HPLC)-UV spectrometry, LC-fluorescence spectrometry, and HPLC-mass spectrometry, are time-consuming, high-cost, and complicated. Here, we present a method for MGO quantitative detection based on far-IR spectral analyses. Our method uses o-phenylenediamine (OPD) to produce a chemical reaction with MGO, which results in multiple fingerprint feature changes associated with the molar ratio of MGO and OPD. We use the linear relationship between MGO concentration and peak intensity of the reaction product to quantitatively determine MGO concentration. The corresponding linear detectable range is 5∼2500 nmol/mL nmol per mL with a correlation coefficient of 0.999. This quantitative method is also tested by blood samples with adjusted MGO concentrations, and shows 95% accuracy with only 30s testing time. Our method provides a fast, simple and economical approach to determining MGO concentration in blood.
甲基乙二醛（MGO）是糖尿病心血管并发症的一个重要病理因素。生物样品中MGO检测的传统方法，如高效液相色谱（HPLC）-紫外光谱法、液相色谱-荧光光谱法和HPLC-质谱法，耗时、成本高且操作复杂。在此，我们提出一种基于远红外光谱分析的MGO定量检测方法。我们的方法使用邻苯二胺（OPD）与MGO发生化学反应，这会导致与MGO和OPD摩尔比相关的多个指纹特征变化。我们利用MGO浓度与反应产物峰强度之间的线性关系来定量测定MGO浓度。相应的线性可检测范围为5∼2500 nmol/mL（每毫升纳摩尔），相关系数为0.999。该定量方法也通过调整MGO浓度的血样进行了测试，在仅30秒的测试时间内显示出95%的准确率。我们的方法为测定血液中MGO浓度提供了一种快速、简单且经济的方法。
