Chong Chen, Simin Li, Gang Wen, Yong Liang, Linbo Wang, Guang Yang, Xin Jin, Hui Li
University of Science and Technology of China, Hefei, Anhui 230041, China.
Jiangsu Key Laboratory of Medical Optics, CAS Center for Excellence in Molecular Cell Science, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu 215163, China.
Biomed Opt Express. 2020 Jan 30;11(2):1203-1215. doi: 10.1364/BOE.382114. eCollection 2020 Feb 1.
We established a four-dimensional (4D) microscopy method using structured illumination for optical axial imaging with an electrically tunable lens. With its fast imaging capability, the dynamics of the cardiovascular system of the zebrafish and cerebral vessels were imaged based on the coverage of two stacks (25 layers) per second with lateral /axial resolutions of 0.6 µm and 1.8 µm, respectively. Time lapse imaging clearly shows the contractile-relaxation response of the beating heart at different cardiac phases and with different mobilities of blood vessels in different regions. This new 4D technique will facilitate imaging of organ function, generation, as well as drug responses in small-sized animals.
我们建立了一种使用结构照明和电可调透镜进行光学轴向成像的四维（4D）显微镜方法。凭借其快速成像能力，基于每秒两层堆栈（25层）的覆盖范围，分别以0.6 µm和1.8 µm的横向/轴向分辨率对斑马鱼心血管系统和脑血管的动力学进行了成像。延时成像清楚地显示了跳动心脏在不同心动周期以及不同区域血管不同活动状态下的收缩-舒张反应。这种新的4D技术将有助于对小型动物的器官功能、生成过程以及药物反应进行成像。
