Hedde Per Niklas, Gratton Enrico
Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California, Irvine, California, USA.
Microsc Res Tech. 2018 Sep;81(9):924-928. doi: 10.1002/jemt.22707. Epub 2016 Jun 24.
Light sheet microscopy is a powerful technique for rapid, three-dimensional fluorescence imaging of large specimen such as drosophila and zebrafish embryos. Yet, beam divergence results in a loss of axial resolution at the periphery of the light sheet. Here, we demonstrate how an electrically tunable lens can be utilized to maintain the minimal, diffraction-limited thickness of the light sheet over a wide field of view (>600 µm) at high frame rates (40 fps). This mode of operation is necessary for the application of fluorescence fluctuation spectroscopy in images. Microsc. Res. Tech. 81:924-928, 2018. © 2016 Wiley Periodicals, Inc.
光片显微镜是一种用于对果蝇和斑马鱼胚胎等大型标本进行快速三维荧光成像的强大技术。然而，光束发散会导致光片边缘轴向分辨率的损失。在这里，我们展示了如何利用电可调透镜在高帧率（40帧/秒）下在宽视场（>600微米）内保持光片的最小衍射极限厚度。这种操作模式对于在图像中应用荧光涨落光谱学是必要的。《显微镜研究与技术》81:924 - 928，2018年。©2016威利期刊公司。
