由可调焦透镜实现的体积结构照明显微镜。
Volumetric structured illumination microscopy enabled by a tunable-focus lens.
作者信息
Hinsdale Taylor, Malik Bilal H, Olsovsky Cory, Jo Javier A, Maitland Kristen C
出版信息
Opt Lett. 2015 Nov 1;40(21):4943-6. doi: 10.1364/OL.40.004943.
Abstract
We present a mechanical-scan-free method for volumetric imaging of biological tissue. The optical sectioning is provided by structured illumination, and the depth of the imaging plane is varied using an electrically tunable-focus lens. We characterize and evaluate the ability of this axial-scanning mechanism in structured illumination microscopy and demonstrate its ability to perform subcellular resolution imaging in oral mucosa ex vivo. The proposed mechanism can potentially convert any wide-field microscope to a 3D-imaging platform without the need for mechanical scanning of imaging optics and/or sample.
摘要
我们提出了一种用于生物组织体积成像的无机械扫描方法。光学切片由结构光照提供，成像平面的深度通过电可调聚焦透镜改变。我们对这种轴向扫描机制在结构光照显微镜中的能力进行了表征和评估，并证明了其在离体口腔黏膜中进行亚细胞分辨率成像的能力。所提出的机制有可能将任何宽视场显微镜转变为一个三维成像平台，而无需对成像光学器件和/或样品进行机械扫描。
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