Bukhari Ishfaq A, Habib Syed S, Alnahedh Alaa, Almutairi Futoon, Alkahtani Lama, Alareek Latefa A, Assiri Ghadah A
Pharmacology, College of Medicine, King Saud University, Riyadh, SAU.
Physiology Department, College of Medicine and King Saud University Medical City, King Saud University, Riyadh, SAU.
Cureus. 2020 Jan 29;12(1):e6815. doi: 10.7759/cureus.6815.
Background Adiposity is firmly linked to a higher incidence of various cardiovascular and metabolic morbidities, including diabetes, hypertension, and thromboembolism. This research study was aimed to verify the association of increased adiposity and hyperreactivity of platelets in obese and non-obese individuals. Methods This cross-sectional study was conducted on 42 subjects aged 18 years and above. Subjects were divided into obese and non-obese groups based on their body mass index (BMI). The data was collected through self-administered questionnaires. All participants underwent body composition analysis. Blood samples were collected from all subjects and taken to the Pharmacology Department for the preparation of platelet-rich plasma (PRP) and poor platelet plasma (PPP). Platelet aggregation was induced by arachidonic acid and was monitored with a Bio/Data multichannel aggregation profiler (Bio/Data Corp., Horsham, PA, USA). Results Significant differences were observed in most parameters, such as fat mass, body fat percentage, free fat mass (FFM), the percentage of trunk fat, total body water, waist-hip ratio (WHR), and basal metabolic rate (BMR) of obese and non-obese subjects. The average percent of platelet aggregation in obese and non-obese subjects was 56.33 ± 15.62 and 59.38 ± 12.62, respectively. The average area under the curve (AUC) for platelet aggregation for both groups was 339.33 ± 191.55 and 342 ± 146.68, respectively. Platelet function was not significantly different and didn't positively correlate with most parameters of the body composition, except WHR, which positively correlated with AUC for platelet function. Conclusion There was no significant direct correlation between adiposity and platelet activation in obese subjects. However, a significant positive correlation of AUC for platelet aggregation with WHR was observed (resistance (r)-value: 0.307, p < 0.05). These findings suggest that WHR could be an effective determinant to assess the risk of thromboembolism in obese individuals.
肥胖与多种心血管和代谢疾病的高发病率密切相关，包括糖尿病、高血压和血栓栓塞。本研究旨在验证肥胖和非肥胖个体中肥胖增加与血小板高反应性之间的关联。
本横断面研究对42名18岁及以上的受试者进行。根据体重指数（BMI）将受试者分为肥胖组和非肥胖组。数据通过自我管理问卷收集。所有参与者均接受身体成分分析。从所有受试者采集血样，并送至药理系制备富血小板血浆（PRP）和贫血小板血浆（PPP）。用花生四烯酸诱导血小板聚集，并用Bio/Data多通道聚集分析仪（Bio/Data Corp.，美国宾夕法尼亚州霍舍姆）进行监测。
在肥胖和非肥胖受试者的大多数参数上观察到显著差异，如脂肪量、体脂百分比、游离脂肪量（FFM）、躯干脂肪百分比、全身水含量、腰臀比（WHR）和基础代谢率（BMR）。肥胖和非肥胖受试者血小板聚集的平均百分比分别为56.33±15.62和59.38±12.62。两组血小板聚集的平均曲线下面积（AUC）分别为339.33±191.55和342±146.68。血小板功能无显著差异，且与身体成分的大多数参数无正相关，但WHR与血小板功能的AUC呈正相关。
肥胖受试者的肥胖与血小板活化之间无显著直接相关性。然而，观察到血小板聚集的AUC与WHR呈显著正相关（相关系数（r）值：0.307，p<0.05）。这些发现表明，WHR可能是评估肥胖个体血栓栓塞风险的有效决定因素。
