Cui Kai, Kang Ning, Banie Lia, Zhou Tie, Liu Tianshu, Wang Bohan, Ruan Yajun, Peng Dongyi, Wang Hsun Shuan, Wang Tianyu, Wang Guifang, Reed-Maldonado Amanda B, Chen Zhong, Lin Guiting, Lue Tom F
Knuppe Molecular Urology Laboratory, Department of Urology, School of Medicine, University of California, San Francisco, CA, USA.
Department of Urology, Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030, China.
Transl Androl Urol. 2019 Oct;8(5):489-500. doi: 10.21037/tau.2019.08.18.
Stress urinary incontinence (SUI) is a common disorder with high prevalence in women across their life span, but there are no non-surgical curative options for the condition. Stem cell-based therapy, especially endogenous stem cell therapy may be a potential treatment method for SUI. The aims of this study are to identify, isolate, and assay the function of urethral striated muscle derived stem/progenitor cells (uMDSCs) and to assess uMDSC response to microenergy acoustic pulses (MAP).
Urethral striated muscle was identified utilizing 3D imaging of solvent organs (3DISCO) and immunofluorescence (IF). uMDSCs were isolated and purified from Zucker Lean (ZL) (ZUC-LEAN) (ZUC-Leprfa 186) rats, with magnetic-activated cell sorting (MACS) and pre-plating methods. The stemness and differentiation potential of the uMDSCs were measured by cell proliferation, EdU, flow cytometry, IF, and Western blot.
Comparison of the cell proliferation assays between MACS and pre-plating reveals the advantage of MACS over pre-plating. In addition, the study reveals that uMDSCs form myotubes when treated with MAP.
The uMDSCs within female rat urethral striated muscle could be a therapeutic target of MAP in managing SUI.
压力性尿失禁（SUI）是一种常见疾病，在女性一生中患病率较高，但目前尚无针对该疾病的非手术治愈方法。基于干细胞的治疗，尤其是内源性干细胞治疗可能是SUI的一种潜在治疗方法。本研究的目的是鉴定、分离和检测尿道横纹肌衍生的干/祖细胞（uMDSCs）的功能，并评估uMDSC对微能量声脉冲（MAP）的反应。
利用溶剂器官三维成像（3DISCO）和免疫荧光（IF）鉴定尿道横纹肌。采用磁激活细胞分选（MACS）和预铺板方法从Zucker Lean（ZL）（ZUC-LEAN）（ZUC-Leprfa 186）大鼠中分离和纯化uMDSCs。通过细胞增殖、EdU、流式细胞术、IF和蛋白质免疫印迹法检测uMDSCs的干性和分化潜能。
MACS和预铺板的细胞增殖试验比较显示MACS优于预铺板。此外，研究表明，uMDSCs在接受MAP处理时会形成肌管。
雌性大鼠尿道横纹肌内的uMDSCs可能是MAP治疗SUI的靶点。
