Department of Infectious Diseases, Huashan Hospital of Fudan University, Shanghai, China.
Front Cell Infect Microbiol. 2020 Feb 18;10:13. doi: 10.3389/fcimb.2020.00013. eCollection 2020.
In this original study, we retrospectively reviewed the cases of nocardiosis diagnosed through culture and next-generation sequencing (NGS) methods between 2014 and 2018 in Huashan Hospital and found out that the latter way can not only improve the detection rate of spp. but also greatly reduce the turnaround time. In addition, by comparing nocardiosis and non-nocardiosis patients both of whose samples had spp. detected by NGS, we found that 's specific reads ranking among top two might be a satisfactory cutoff value for clinical diagnosis of the disease. Our study introduced the promising value of the NGS method in the rapid diagnosis of nocardiosis.
在这项原始研究中，我们回顾性地分析了华山医院在 2014 年至 2018 年间通过培养和下一代测序（NGS）方法诊断的诺卡氏菌病病例，发现后者不仅可以提高 spp.的检出率，还可以大大缩短周转时间。此外，通过比较 NGS 检测到 spp.的诺卡氏菌病患者和非诺卡氏菌病患者，我们发现“s 特定读长排名前两位可能是临床诊断该病的一个满意的截断值。我们的研究介绍了 NGS 方法在快速诊断诺卡氏菌病方面的有前景的价值。
