Berny Clément, Le Fèvre Raphael, Guyot François, Blondeau Karine, Guizonne Christine, Rousseau Emilie, Bayan Nicolas, Alphandéry Edouard
Nanobacterie SARL, Paris, France.
Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette, France.
Front Bioeng Biotechnol. 2020 Feb 18;8:16. doi: 10.3389/fbioe.2020.00016. eCollection 2020.
We report the synthesis in large quantity of highly pure magnetosomes for medical applications. For that, magnetosomes are produced by MSR-1 magnetotactic bacteria using minimal growth media devoid of uncharacterized and toxic products prohibited by pharmaceutical regulation, i.e., yeast extract, heavy metals different from iron, and carcinogenic, mutagenic and reprotoxic agents. This method follows two steps, during which bacteria are first pre-amplified without producing magnetosomes and are then fed with an iron source to synthesize magnetosomes, yielding, after 50 h of growth, an equivalent OD of ~8 and 10 mg of magnetosomes in iron per liter of growth media. Compared with magnetosomes produced in non-minimal growth media, those particles have lower concentrations in metals other than iron. Very significant reduction or disappearance in magnetosome composition of zinc, manganese, barium, and aluminum are observed. This new synthesis method paves the way towards the production of magnetosomes for medical applications.
我们报告了用于医学应用的大量高纯度磁小体的合成。为此，磁小体由MSR-1趋磁细菌利用不含制药法规禁止的未表征和有毒产物的基本生长培养基产生，即酵母提取物、除铁以外的重金属以及致癌、致突变和生殖毒性剂。该方法分两步进行，在此期间，细菌首先在不产生磁小体的情况下进行预扩增，然后用铁源喂养以合成磁小体，生长50小时后，每升生长培养基产生约8的等效OD值和10毫克铁含量的磁小体。与在非基本生长培养基中产生的磁小体相比，这些颗粒中铁以外的金属浓度较低。观察到锌、锰、钡和铝在磁小体组成中的含量大幅降低或消失。这种新的合成方法为医学应用磁小体的生产铺平了道路。
