Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran.
Stem Cells and Regenerative Medicine Innovation Center, Kerman University of Medical Sciences, Kerman, Iran.
Sci Rep. 2024 Aug 29;14(1):20141. doi: 10.1038/s41598-024-70896-4.
Many notable applications have been described for magnetic nanoparticles in delivery of diverse drugs and bioactive compounds into cells, magnetofection for the treatment of cancer, photodynamic therapy, photothermal therapy, and magnetic particle imaging (MPI). In response to the growing demand for magnetic nanoparticles for drug delivery or biomedical imaging applications, more effective and eco-friendly methodologies are required for large-scale biosynthesis of this nanoparticles. The major challenge in the large-scale biomedical application of magnetic nanoparticles lies in its low efficiency and optimization of nanoparticle production can address this issue. In the current study, a prediction model is suggested by the fractional factorial designs. The present study aims to optimize culture media components for improved growth and iron uptake of this strain. The result of optimization for iron uptake by the strain ASFS1 is to increase the production of magnetic nanoparticles by this strain for biomedical applications in the future. In the present study, design of experiment method was used to probe the effects of some key medium components (yeast extract, tryptone, FeSO, Na-EDTA, and FeCl) on Fe content in biomass and dried biomass of strain ASFS1. A 2 fractional factorial design showed that Na-EDTA, FeCl, yeast extract-tryptone interaction, and FeSO-Na-EDTA interaction were the most parameters on Fe content in biomass within the experimented levels (p < 0.05), while yeast extract, FeCl, and yeast extract-tryptone interaction were the most significant factors within the experimented levels (p < 0.05) to effect on dried biomass of strain ASFS1. The optimum culture media components for the magnetic nanoparticles production by strain ASFS1 was reported to be 7.95 g L of yeast extract, 5 g L of tryptone, 75 μg mL of FeSO, 192.3 μg mL of Na-EDTA and 150 μg mL of FeCl which was theoretically able to produce Fe content in biomass (158 μg mL) and dried biomass (2.59 mg mL) based on the obtained for medium optimization. Using these culture media components an experimental maximum Fe content in biomass (139 ± 13 μg mL) and dried biomass (2.2 ± 0.2 mg mL) was obtained, confirming the efficiency of the used method.
许多著名的应用已经被描述为在细胞内递送电介质和生物活性化合物的磁性纳米粒子，用于治疗癌症的磁转染、光动力疗法、光热疗法和磁性粒子成像（MPI）。为了满足对用于药物输送或生物医学成像应用的磁性纳米粒子不断增长的需求，需要更有效和环保的方法来大规模生物合成这些纳米粒子。在磁性纳米粒子的大规模生物医学应用中，主要的挑战在于其效率低下，而优化纳米粒子的生产可以解决这个问题。在本研究中，通过分数阶实验设计提出了一个预测模型。本研究旨在优化培养基成分，以提高该菌株的生长和铁摄取率。优化菌株 ASFS1 的铁摄取率的结果是提高该菌株未来用于生物医学应用的磁性纳米粒子的产量。在本研究中，使用实验设计方法来探测一些关键培养基成分（酵母提取物、胰蛋白胨、FeSO、Na-EDTA 和 FeCl）对 ASFS1 菌株生物量和干生物量中铁含量的影响。2 分数阶实验设计表明，在实验范围内，Na-EDTA、FeCl、酵母提取物-胰蛋白胨相互作用和 FeSO-Na-EDTA 相互作用是对生物量中铁含量影响最大的参数（p<0.05），而酵母提取物、FeCl 和酵母提取物-胰蛋白胨相互作用是对干生物量影响最大的因素（p<0.05）。报道称，ASFS1 菌株生产磁性纳米粒子的最佳培养基成分是 7.95 g/L 酵母提取物、5 g/L 胰蛋白胨、75μg/mL FeSO、192.3μg/mL Na-EDTA 和 150μg/mL FeCl，理论上能够产生生物量中的铁含量（158μg/mL）和干生物量（2.59mg/mL）基于获得的培养基优化。使用这些培养基成分，实验获得了生物量中最大的铁含量（139±13μg/mL）和干生物量（2.2±0.2mg/mL），证实了所用方法的效率。
