中年女性类风湿关节炎相关间质性肺病患者外周血单个核细胞中差异表达的长链非编码 RNA。
Differentially expressed lncRNAs in peripheral blood mononuclear cells from middle-aged female patients with rheumatoid arthritis-associated interstitial lung disease.
机构信息
Department of Rheumatology, Shandong University Qilu Hospital, 107 Wenhua Xi Road, Jinan, 250012, Shandong Province, China.
Department of Rheumatology, Liaocheng People's Hospital, Liaocheng, 252000, China.
出版信息
Clin Rheumatol. 2020 Aug;39(8):2281-2289. doi: 10.1007/s10067-020-04977-w. Epub 2020 Mar 5.
OBJECTIVES
Interstitial lung disease (ILD) is frequently associated with rheumatoid arthritis (RA) and has a large impact on the prognosis of RA, particularly among females, with an increased prevalence and severity compared with that of males. Here, we aimed to investigate the lncRNA profiles in peripheral blood mononuclear cells (PBMCs) from middle-aged female patients with RA-ILD to determine whether they could help diagnose RA-ILD.
METHOD
We collected PBMCs from middle-aged female healthy controls, and RA and RA-ILD patients, excluding those with known risk factors of RA-ILD, such as being elderly or male, smoking, and having a history of other diseases. Then, a microarray analysis was applied to profile the lncRNA and mRNA levels in 3 pairs of samples. qPCR was performed to evaluate the candidate lncRNAs from 20 participants of each group.
RESULTS
The expression levels of NR_002819, NR_038935, and ENST00000603415 were significantly increased in the RA-ILD group, while the expression level of ENST00000560199 was significantly decreased. As risk factors for RA-ILD, the area under the curve (AUC) values of NR_002819, NR_038935, and ENST00000603415 were 0.858, 0.704 (medium diagnostic accuracy), and 0.976 (high diagnostic accuracy), respectively. As a protective factor for RA-ILD, the AUC of ENST00000560199 was 0.853(medium diagnostic accuracy).
CONCLUSIONS
To the best of our knowledge, this is the first study of lncRNA profiles in RA-ILD. The expression levels of NR_002819 (MALAT1), NR_038935, ENST00000603415, and ENST00000560199 were significantly different in the RA-ILD group and could be potential biomarkers for the assessment and diagnosis of middle-aged female RA-ILD patients. Key Points • The expression profile of lncRNAs in PBMCs from RA-ILD patients was evaluated. • NR_002819, NR_038935, and ENST00000603415 increased in RA-ILD patients. •ENST00000560199 decreased in RA-ILD patients. • The 4 lncRNAs might be potential biomarkers for diagnosis of RA-ILD.
目的
间质性肺病（ILD）常与类风湿关节炎（RA）相关，对 RA 的预后有很大影响，尤其是在女性中，其患病率和严重程度均高于男性。本研究旨在探讨中年女性 RA-ILD 患者外周血单个核细胞（PBMC）中的 lncRNA 谱，以确定其是否有助于诊断 RA-ILD。
方法
我们收集了中年女性健康对照组、RA 组和 RA-ILD 组的 PBMC，排除了已知的 RA-ILD 危险因素，如高龄或男性、吸烟和其他疾病史。然后，对 3 对样本进行了微阵列分析，以分析 lncRNA 和 mRNA 水平。对每组 20 名参与者进行 qPCR 评估候选 lncRNA。
结果
NR_002819、NR_038935 和 ENST00000603415 的表达水平在 RA-ILD 组中显著升高，而 ENST00000560199 的表达水平显著降低。NR_002819、NR_038935 和 ENST00000603415 的曲线下面积（AUC）值分别为 0.858、0.704（中等诊断准确性）和 0.976（高诊断准确性），作为 RA-ILD 的危险因素。作为 RA-ILD 的保护因素，ENST00000560199 的 AUC 值为 0.853（中等诊断准确性）。
结论
据我们所知，这是 RA-ILD 中 lncRNA 谱的首次研究。NR_002819（MALAT1）、NR_038935、ENST00000603415 和 ENST00000560199 的表达水平在 RA-ILD 组中存在显著差异，可能是评估和诊断中年女性 RA-ILD 患者的潜在生物标志物。
关键点
• 评估了 RA-ILD 患者 PBMC 中 lncRNA 的表达谱。
• NR_002819、NR_038935 和 ENST00000603415 在 RA-ILD 患者中增加。
• ENST00000560199 在 RA-ILD 患者中减少。
• 这 4 个 lncRNA 可能是 RA-ILD 诊断的潜在生物标志物。
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