Huang H F, Yang C S, Meyenhofer M, Gould S, Boccabella A V
Department of Anatomy, University of Medicine and Dentistry of New Jersey, New Jersey Medical School, Newark.
Acta Anat (Basel). 1988;133(1):10-5. doi: 10.1159/000146606.
The relationship between the intactness of sustentacular (Sertoli) cell tight junctions and the status of spermatogenesis was examined in rats fed a vitamin-A-deficient diet after weaning (VAD rats). Both serum and testicular retinol concentrations of the VAD rats declined to a nadir by 80 days of age. At this time, it was observed that Sertoli cell tight junctions of the VAD animals were intact and complete spermatogenesis was maintained. Leakage in Sertoli cell tight junctions, as demonstrated by the presence of lanthanum in the adluminal compartment of the seminiferous epithelium, was first observed in 90-day-old VAD rats. Severe regression of spermatogenic cells was noted in 100-day or older VAD animals. These results suggest that severe germ cell loss observed during chronic vitamin A deficiency may result from abnormal intratubular environment due to the disruption of the blood-testis barrier.
在断奶后喂食维生素A缺乏饮食的大鼠（VAD大鼠）中，研究了支持细胞（塞尔托利氏细胞）紧密连接的完整性与精子发生状态之间的关系。VAD大鼠的血清和睾丸视黄醇浓度在80日龄时均降至最低点。此时，观察到VAD动物的支持细胞紧密连接完整，精子发生维持正常。生精上皮管腔侧隔室中镧的存在表明支持细胞紧密连接出现渗漏，这首先在90日龄的VAD大鼠中观察到。在100日龄及以上的VAD动物中，生精细胞出现严重退化。这些结果表明，慢性维生素A缺乏期间观察到的严重生殖细胞损失可能是由于血睾屏障破坏导致的异常管内环境所致。
