Hirota Y, Kurohmaru M, Matsumoto M, Hayashi Y
Department of Veterinary Anatomy, Faculty of Agriculture, University of Tokyo, Japan.
J Vet Med Sci. 1994 Jun;56(3):439-42. doi: 10.1292/jvms.56.439.
The effect of vitamin A deficiency and vitamin A replacement on spermatogenesis in golden hamsters was studied using a light microscope. Male golden hamsters were fed a vitamin A deficient (VAD) diet from 3 weeks of age. Hamsters with a VAD diet reached maximum body weight at about 13 weeks. After 17 weeks, the body weight of the hamsters began to decrease. When their body weight decreased to 70 g, only Sertoli cells, spermatogonia, and a few spermatocytes were present within the seminiferous tubules. Administering retinol acetate (vitamin A) combined with a conventional diet to the VAD hamsters induced a reinitiation of spermatogenesis with stage-synchronization. At 9, 10, and 11 weeks after vitamin A replacement, the testes with active spermatogenesis possessed only a few successive stages of the seminiferous epithelial cycle.
利用光学显微镜研究了维生素A缺乏和维生素A补充对金黄地鼠精子发生的影响。雄性金黄地鼠从3周龄开始喂食维生素A缺乏（VAD）饮食。食用VAD饮食的地鼠在约13周时达到最大体重。17周后，地鼠体重开始下降。当它们的体重降至70克时，生精小管内仅存在支持细胞、精原细胞和少数精母细胞。给VAD地鼠喂食醋酸视黄醇（维生素A）并搭配常规饮食可诱导精子发生重新启动并实现阶段同步。在维生素A补充后的第9、10和11周，精子发生活跃的睾丸仅具有生精上皮周期的几个连续阶段。
