Division of Virology and Zoonoses, ICMR-National Institute of Research in Tribal Health, Jabalpur, Madhya Pradesh, India.
Maximum Containment Laboratory, ICMR-National Institute of Virology, Pune, Maharashtra, India.
Indian J Med Res. 2020 Jan;151(1):87-92. doi: 10.4103/ijmr.IJMR_204_19.
BACKGROUND & OBJECTIVES: Chikungunya (CHIK) is a neglected, re-emerging arboviral disease. Limited information on CHIK-confirmed cases during interepidemic period is available from India. This surveillance study was conducted in Madhya Pradesh (MP), India, during the years 2016-2017, to provide information about CHIK cases.
Blood samples collected from patients suspected having CHIK were tested by immunoglobulin (Ig) IgM ELISA or real time reverse transcription-polymerase chain reaction (rRT-PCR) for the detection of CHIK virus (CHIKV)-specific IgM antibodies or viral RNA, respectively. Partial envelope-1 gene sequencing was done. Clinical and demographic data were collected and analyzed.
Of the 4019 samples tested, 494 (12.2%) were found positive for CHIKV infection. The positivity was detected in both rural and urban areas. The mean age of CHIK-positive cases was 33.12±18.25 yr. Headache and joint pain were the most prominent symptoms, 34.6 per cent (171/494) of the CHIK cases required hospitalization and six patients with CHIKV infection died. The East/Central/South African genotype of CHIKV was found to be circulating in the study area.
INTERPRETATION & CONCLUSIONS: Our study recorded a higher CHIK positivity during 2016-2017 in comparison to earlier reports from MP, India. A high proportion of CHIK cases required hospitalization and deaths were also reported, which indicated the severity of the disease in the study area. In-depth molecular analysis of the virus and other risk factors is essential to understand the trends in disease severity.
基孔肯雅热（CHIK）是一种被忽视的、重新出现的虫媒病毒病。印度在流行间歇期有关于确诊 CHIK 病例的信息有限。本监测研究于 2016-2017 年在印度中央邦（MP）进行，旨在提供有关 CHIK 病例的信息。
采集疑似患有 CHIK 的患者的血液样本，通过免疫球蛋白（Ig）IgM ELISA 或实时逆转录-聚合酶链反应（rRT-PCR）检测 CHIK 病毒（CHIKV）特异性 IgM 抗体或病毒 RNA。进行部分包膜-1 基因测序。收集并分析临床和人口统计学数据。
在检测的 4019 个样本中，有 494 个（12.2%）被检测出 CHIKV 感染呈阳性。在农村和城市地区都检测到了阳性。CHIK 阳性病例的平均年龄为 33.12±18.25 岁。头痛和关节痛是最突出的症状，494 例 CHIK 病例中有 34.6%（171/494）需要住院治疗，有 6 例 CHIKV 感染患者死亡。研究区域内循环的 CHIKV 为东/中非基因型。
与印度 MP 之前的报告相比，我们的研究在 2016-2017 年记录了更高的 CHIK 阳性率。很大比例的 CHIK 病例需要住院治疗，也有死亡报告，这表明研究区域疾病的严重程度。深入分析病毒和其他风险因素的分子分析对于了解疾病严重程度的趋势至关重要。
