Barde Pradip V, Shukla Mohan K, Bharti Praveen K, Kori Bhupesh K, Jatav Jayant K, Singh Neeru
Regional Medical Research Centre for Tribals (ICMR), Jabalpur, Madhya Pradesh, India.
Netaji Subhash Chandra Bose Medical Collage, Jabalpur, Madhya Pradesh, India.
WHO South East Asia J Public Health. 2014 Jan-Mar;3(1):36-40. doi: 10.4103/2224-3151.206881.
Dengue and chikungunya present with very similar signs and symptoms in the initial stage of illness and so it is difficult to distinguish them clinically. Both are transmitted by Aedes aegypti and Aedes albopictus mosquitoes. This study was conducted with the aim to explore the co-circulation of dengue and chikungunya viruses in central India.
Samples from suspected dengue cases were subjected to dengue immunoglobulin M (lgM) enzyme-linked immunosorbent assay (ELISA) and dengue-negative samples were tested with chikungunya-specific IgM ELISA. The samples collected in acute phase of illness were tested by nested reverse transcription polymerase chain reaction (nRT-PCR). Chikungunya virus (CHIKV) sequences were analysed to determine their genotype.
Of 138 samples screened for dengue, 21 (15.2%) were positive, and of 119samples screened for chikungunya, 13 (10.9%) were positive. Dengue viruses 1 and 4 were found co-circulating with chikungunya virus in Jabalpur, central India. The chikungunya virus detected belonged to the East Central South African genotype.
Accurate and timely diagnosis would help in patient management and use of resources. It is advocated to simultaneously test samples for these two diseases in endemic areas. This will also aid in understanding the epidemiology of chikungunya.
登革热和基孔肯雅热在疾病初期表现出非常相似的体征和症状，因此在临床上很难区分。两者均由埃及伊蚊和白纹伊蚊传播。本研究旨在探索印度中部登革热病毒和基孔肯雅热病毒的共同流行情况。
对疑似登革热病例的样本进行登革热免疫球蛋白M（IgM）酶联免疫吸附测定（ELISA），对登革热阴性样本进行基孔肯雅热特异性IgM ELISA检测。对疾病急性期采集的样本进行巢式逆转录聚合酶链反应（nRT-PCR）检测。对基孔肯雅热病毒（CHIKV）序列进行分析以确定其基因型。
在筛查的138份登革热样本中，21份（15.2%）呈阳性，在筛查的119份基孔肯雅热样本中，13份（10.9%）呈阳性。在印度中部贾巴尔普尔发现登革热病毒1型和4型与基孔肯雅热病毒共同流行。检测到的基孔肯雅热病毒属于东中南非洲基因型。
准确及时的诊断有助于患者管理和资源利用。提倡在流行地区同时对这两种疾病的样本进行检测。这也将有助于了解基孔肯雅热的流行病学情况。
