Seber S, Sirin D Y, Yetisyigit T, Bilgen T
Department of Medical Oncology, Faculty of Medicine, Tekirdağ Namık Kemal University, Tekirdağ, Turkey.
Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Tekirdağ Namık Kemal University, Tekirdağ, Turkey.
Niger J Clin Pract. 2020 Mar;23(3):386-391. doi: 10.4103/njcp.njcp_80_19.
Piperlongumine (PL) is an alkaloid derived from the edible pepper (Piper longum L) and it has been described to have various biologic activities including anticancer effects. Our aim in this study was to assess the cytotoxic role of PL on a cervical cancer cell line (HeLa) and to evaluate the effects of PL/doxorubicin and PL/paclitaxel combination therapies on apoptotic cancer cell death.
The cytotoxicity, IC50 doses by MTT assay confirmed by fluorescent imaging, and apoptotic cell rates by Annexin V staining using flow cytometry were determined for PL, doxorubicin, paclitaxel, and for their combinations.
It was shown that the PL by itself induced the apoptosis in HeLa cells. PL in combination with doxorubicin and paclitaxel increased apoptotic cell death compared to either chemotherapeutic agent alone.
We conclude that the PL inhibits cancer cell growth by inducing apoptosis and has a potential anticancer activity in cervical cancer, especially when combined with doxorubicin and paclitaxel.
胡椒碱（PL）是一种从食用胡椒（荜茇）中提取的生物碱，据报道具有多种生物学活性，包括抗癌作用。本研究的目的是评估PL对宫颈癌细胞系（HeLa）的细胞毒性作用，并评估PL/阿霉素和PL/紫杉醇联合疗法对癌细胞凋亡死亡的影响。
通过MTT法测定PL、阿霉素、紫杉醇及其组合的细胞毒性、荧光成像确认的IC50剂量，以及使用流式细胞术通过膜联蛋白V染色测定凋亡细胞率。
结果表明，PL本身可诱导HeLa细胞凋亡。与单独使用任何一种化疗药物相比，PL与阿霉素和紫杉醇联合使用可增加凋亡细胞死亡。
我们得出结论，PL通过诱导凋亡抑制癌细胞生长，在宫颈癌中具有潜在的抗癌活性, 尤其是与阿霉素和紫杉醇联合使用时。
