长非编码 RNA FENDRR 抑制皮肤恶性黑素瘤细胞的迁移和侵袭。
Long non-coding RNA FENDRR inhibits migration and invasion of cutaneous malignant melanoma cells.
机构信息
Department of Dermatology, Guizhou Provincial People's Hospital, Guiyang, Guizhou 550002, China.
Department of Information, Guizhou Province Hospital of Traditional Chinese Medicine, Guiyang, Guizhou 550001, China.
出版信息
Biosci Rep. 2020 Mar 27;40(3). doi: 10.1042/BSR20191194.
The present study aimed to investigate the effects of lncRNA FENDRR on the migration and invasion of malignant melanoma (MM) cells. The expression levels of FENDRR in MM tissues and MM cell lines were detected using qRT-PCR, followed by construction of FENDRR-knocked down and overexpressed stable cells. Then the effects of FENDRR on cell proliferation, migration and invasion were detected using MTT assay and Transwell assay. The protein expression levels of matrix metallopeptidase 2 (MMP2), MMP9, and related factors in JNK/c-Jun pathway were detected using Western blot. FENDRR was down-regulated in MM tissues and cell lines. Besides, its expression levels in different MM cells were diverse. Knockdown of FENDRR facilitated MM cells proliferation, migration and invasion in A375 cells, while overexpressing FENDRR had reverse results. In addition, MMPs and JNK/c-Jun pathway involved in the FENDRR-mediated regulation of MM cell proliferation, migration and invasion. Our results demonstrated that FENDRR mediated the metastasis phenotype of MM cells by inhibiting the expressions of MMP2 and MMP9 and antagonizing the JNK/c-Jun pathway.
本研究旨在探讨长非编码 RNA FENDRR 对恶性黑色素瘤（MM）细胞迁移和侵袭的影响。采用 qRT-PCR 检测 MM 组织和 MM 细胞系中 FENDRR 的表达水平，构建 FENDRR 敲低和过表达稳定细胞。然后采用 MTT 法和 Transwell 法检测 FENDRR 对细胞增殖、迁移和侵袭的影响。采用 Western blot 检测 JNK/c-Jun 通路中基质金属蛋白酶 2（MMP2）、MMP9 及相关因子的蛋白表达水平。FENDRR 在 MM 组织和细胞系中下调。此外，其在不同 MM 细胞中的表达水平也不同。在 A375 细胞中敲低 FENDRR 促进 MM 细胞的增殖、迁移和侵袭，而过表达 FENDRR 则有相反的结果。此外，MMPs 和 JNK/c-Jun 通路参与了 FENDRR 介导的 MM 细胞增殖、迁移和侵袭的调节。我们的结果表明，FENDRR 通过抑制 MMP2 和 MMP9 的表达以及拮抗 JNK/c-Jun 通路来介导 MM 细胞的转移表型。
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