震颤小鼠血清和层粘连蛋白对培养的少突胶质细胞增殖和分化的影响。
Effects of trembler mouse serum and laminin on oligodendrocyte proliferation and differentiation in culture.
作者信息
Hantaz-Ambroise D, Vigny M, Alliot F
机构信息
INSERM U.153, Paris, France.
出版信息
Int J Dev Neurosci. 1988;6(3):289-99. doi: 10.1016/0736-5748(88)90009-3.
Oligodendrocytes in primary cultures derived from rat embryo spinal cord were examined in control medium and in Trembler mouse serum (TMS)-supplemented medium. The oligodendrocytes were identified on the basis of the synthesis and surface expression of galactocerebrosides revealed by a monoclonal antibody directed against this component. We noticed two effects of TMS compared to control mouse serum. First, our results revealed that in TMS medium there is a mitogenic response of galactocerebroside (GalC)-positive cells. Second, in the presence of TMS, oligodendrocytes do not develop processes as they do in the presence of normal mouse serum. When laminin, a basal lamina component was added to TMS medium, GalC+ oligodendrocytes decreased in number and differentiation was normal. Possible explanations of the effects of TMS and laminin on oligodendrocyte proliferation and differentiation are discussed.
在来自大鼠胚胎脊髓的原代培养物中，对少突胶质细胞在对照培养基和添加了震颤小鼠血清（TMS）的培养基中进行了检查。根据针对半乳糖脑苷脂的单克隆抗体所揭示的半乳糖脑苷脂的合成和表面表达情况来鉴定少突胶质细胞。与对照小鼠血清相比，我们注意到TMS有两种作用。首先，我们的结果显示，在TMS培养基中，半乳糖脑苷脂（GalC）阳性细胞有促有丝分裂反应。其次，在有TMS存在的情况下，少突胶质细胞不像在正常小鼠血清存在时那样形成突起。当向TMS培养基中添加层粘连蛋白（一种基底膜成分）时，GalC+少突胶质细胞数量减少且分化正常。文中讨论了TMS和层粘连蛋白对少突胶质细胞增殖和分化作用的可能解释。
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