Grynpas M D, Hunter G K
Department of Pathology, University of Toronto, Canada.
J Bone Miner Res. 1988 Apr;3(2):159-64. doi: 10.1002/jbmr.5650030206.
The relation between bone mineralization and glycosaminoglycans (GAG) was investigated in newborn and mature rabbit diaphyseal bone. Using the density fractionation technique, the bone was separated into fractions of increasing density from 1.4 to 2.3 grams/ml. Each fraction was analyzed by X-ray diffraction to determine the average crystal size. The GAG content of each fraction and of the unfractionated bone was determined by direct extraction and on a few fractions by sequential extraction in guanidine hydrochloride and guanidine/EDTA. There was a decrease in the GAG content with animal age and with increasing fraction density in the newborn rabbit. In one overlapping fraction (2.0-2.1 grams/ml), the GAG content was twice as high in the newborn as in the mature animal. Finally, the crystal size substantially increased from newborn to mature rabbits. Therefore, calcification and maturation of bone is associated with a decrease in the proteoglycan content of the organic matrix.
研究了新生和成年兔骨干骨中骨矿化与糖胺聚糖（GAG）之间的关系。采用密度分级技术，将骨分离成密度从1.4到2.3克/毫升递增的级分。通过X射线衍射分析每个级分，以确定平均晶体尺寸。通过直接提取以及对少数级分用盐酸胍和胍/乙二胺四乙酸顺序提取来测定每个级分和未分级骨的GAG含量。新生兔的GAG含量随动物年龄和级分密度增加而降低。在一个重叠级分（2.0 - 2.1克/毫升）中，新生动物的GAG含量是成年动物的两倍。最后，从新生兔到成年兔，晶体尺寸大幅增加。因此，骨的钙化和成熟与有机基质中蛋白聚糖含量的降低有关。
