Nicholson G C, Yumita S, Moseley J M, Yates A J, Martin T J
University of Melbourne, Department of Medicine, Repatriation General Hospital, Heidelberg, Australia.
J Bone Miner Res. 1988 Apr;3(2):181-4. doi: 10.1002/jbmr.5650030209.
Recently we have shown that forskolin acts synergistically with calcitonin on stimulation of cyclic AMP (cAMP) production in isolated rat osteoclasts and now show that forskolin also augments later physiological responses to calcitonin. The sensitivity of calcitonin inhibition of cytoplasmic spreading in isolated rat osteoclasts was increased 3.5-fold by simultaneous treatment with 10(-7) M forskolin, which alone had no effect on the response. In weanling rats the threshold of the hypocalcemic response to salmon calcitonin was reduced fivefold by simultaneous treatment with 200 micrograms/kg forskolin i.v., which alone did not influence the plasma calcium. These results provide further evidence that calcitonin-induced inhibition of osteoclast function is mediated by elevation of cAMP levels and suggest that forskolin could be used to augment the therapeutic effects of calcitonin.
最近我们发现，福司可林与降钙素协同作用，刺激离体大鼠破骨细胞中环磷酸腺苷（cAMP）的产生，并且现在表明福司可林还增强了对降钙素的后期生理反应。在离体大鼠破骨细胞中，通过与10^(-7) M福司可林同时处理，降钙素抑制细胞质铺展的敏感性提高了3.5倍，而福司可林单独处理对该反应没有影响。在断奶大鼠中，通过静脉注射200微克/千克福司可林同时处理，对鲑鱼降钙素的低钙血症反应阈值降低了五倍，而福司可林单独处理不影响血浆钙。这些结果进一步证明，降钙素诱导的破骨细胞功能抑制是由cAMP水平升高介导的，并表明福司可林可用于增强降钙素的治疗效果。
