State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation), Shenzhen, China.
Key Laboratory of Food Biological Safety Control, Shenzhen, China.
Am J Chin Med. 2020;48(2):463-485. doi: 10.1142/S0192415X2050024X. Epub 2020 Mar 5.
Oxidative stress (OS) is the common mechanism for age-related diseases. The co-occurrence of osteoporosis (OP) and cardiovascular disease (CVD) in postmenopausal women makes it warranted to find a holistic approach for treatment of multiple diseases or conditions. The rhizome of Hort. (CX), which has high anti-oxidant properties and is widely used for CVD treatment in China, might be the potential candidate. In the present study, CX ethanol extract (CXE) was applied to HO induced MG63 cells to study its effects and mechanisms on osteoblastogenesis against OS. CXE was then administered to six-month-old Sprague Dawley sham or ovariectomized (OVX) rats fed either a low saturated fat-sucrose (LFS) or a high fat-sucrose (HFS) diet for 12 weeks, to confirm its anti-osteoporotic effects. The results demonstrated that CXE directly improved proliferation and differentiation in an HO-induced osteoblast cell model by attenuating cellular reactive oxygen species levels and inhibiting osteoblast apoptosis via PI3K/Akt signaling pathway. CXE significantly improved bone properties as revealed by the increase in trabecular bone mineral density and decrease in trabecular separation at proximal metaphysis of the tibia (PT) in HFS-fed OVX rats but not in LFS-fed OVX rats. CXE ameliorated dyslipidemia, greatly reduced lipid deposition and malondialdehyde levels, improved activities of superoxide dismutase, catalase and glutathione peroxidase in the livers of HFS-fed OVX rats. In conclusion, CXE could favor osteoblastogenesis against OS. The ability of CXE to reduce bone loss in HFS-fed OVX rats was associated with its abilities to correct dyslipidemia, and reduce lipid deposition and OS levels.
氧化应激（OS）是与年龄相关疾病的共同机制。绝经后妇女中骨质疏松症（OP）和心血管疾病（CVD）的同时发生使得有必要寻找一种综合方法来治疗多种疾病或病症。在中国，广泛用于 CVD 治疗的姜科植物根茎（CX）具有高抗氧化特性，可能是潜在的候选药物。在本研究中，将 CX 乙醇提取物（CXE）应用于 HO 诱导的 MG63 细胞，以研究其对 OS 下成骨细胞发生的影响和机制。然后，将 CXE 给予 6 个月大的 Sprague Dawley 假手术或卵巢切除（OVX）大鼠，这些大鼠分别喂食低饱和脂肪-蔗糖（LFS）或高脂肪-蔗糖（HFS）饮食 12 周，以确认其抗骨质疏松作用。结果表明，CXE 通过降低细胞活性氧水平和抑制成骨细胞凋亡来直接改善 HO 诱导的成骨细胞模型中的增殖和分化，其通过 PI3K/Akt 信号通路实现。在 HFS 喂养的 OVX 大鼠中，CXE 显著改善了骨特性，表现为胫骨近端干骺端（PT）的小梁骨密度增加和小梁分离减少，但在 LFS 喂养的 OVX 大鼠中没有改善。CXE 改善了血脂异常，大大减少了肝脏中的脂质沉积和丙二醛水平，提高了 HFS 喂养的 OVX 大鼠肝脏中超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性。总之，CXE 可以有利于成骨细胞发生以对抗 OS。CXE 减少 HFS 喂养的 OVX 大鼠骨丢失的能力与其纠正血脂异常、减少脂质沉积和 OS 水平的能力有关。
