Hunan University of Traditional Chinese Medicine, Changsha, 410208, China.
Hunan Academy of Chinese Medicine, No. 58 Lushan Road, Yuelu District, Changsha, Hunan Province, 410006, China.
J Orthop Surg Res. 2024 Jul 5;19(1):393. doi: 10.1186/s13018-024-04890-x.
To aim of this study is to assess the mechanism through which Desertliving Cistanche modulates the PI3K/AKT signaling pathway in the treatment of hyperlipidemic osteoporosis in ovariectomized rats.
We randomly assigned specific-pathogen-free (SPF) rats into five groups (n = 10 per group). The normal control group received a standard diet, while the model group, atorvastatin group, diethylstilbestrol group, and treatment group were fed a high-fat diet. Four weeks later, bilateral ovariectomies were conducted, followed by drug interventions. After six weeks of treatment, relevant indicators were compared and analyzed.
Compared to the normal control group, rats in the model group exhibited blurred trabecular morphology, disorganized osteocytes, significantly elevated levels of bone-specific alkaline phosphatase (BALP), bone Gla-protein (BGP), total cholesterol (TC), tumor necrosis factor-α (TNF-α), and receptor activator of NF-κB ligand (RANKL). Also, the model group revealed significantly reduced levels of ultimate load, fracture load, estradiol (E2), bone mineral density (BMD), osteoprotegerin (OPG), and phosphoinositide 3-kinase (PI3K) and protein kinase B (Akt) in femoral tissue. The atorvastatin group presented with higher TC and TNF-α levels compared to the normal control group. Conversely, the treatment group demonstrated enhanced trabecular morphology, denser structure, smaller bone marrow cavities, and reduced BALP, BGP, TC, TNF-α, and RANKL levels. Furthermore, the treatment group exhibited higher levels of E2, BMD, OPG, and PI3K and Akt in bone tissue compared to the model group. The treatment group also had lower TC and TNF-α levels than the atorvastatin group. Biomechanical analysis indicated that after administration of Desertliving Cistanche, the treatment group had reduced body mass, increased ultimate and fracture load of the femur, denser bone structure, smaller bone marrow cavities, and altered periosteal arrangement compared to the model group.
Our study revealed that Desertliving Cistanche demonstrated significant efficacy in preventing and treating postmenopausal hyperlipidemic osteoporosis in rats.
本研究旨在评估荒漠肉苁蓉通过调节 PI3K/AKT 信号通路治疗去卵巢大鼠高脂性骨质疏松的作用机制。
我们将 SPF 大鼠随机分为五组（每组 10 只）。正常对照组给予标准饮食，模型组、阿托伐他汀组、己烯雌酚组和治疗组给予高脂饮食。四周后行双侧卵巢切除术，然后进行药物干预。治疗 6 周后，比较和分析相关指标。
与正常对照组相比，模型组大鼠的骨小梁形态模糊，骨细胞排列紊乱，骨碱性磷酸酶（BALP）、骨钙素（BGP）、总胆固醇（TC）、肿瘤坏死因子-α（TNF-α）和核因子-κB 受体激活剂配体（RANKL）水平显著升高。同时，模型组大鼠股骨组织中最终载荷、骨折载荷、雌二醇（E2）、骨密度（BMD）、骨保护素（OPG）、磷酸肌醇 3-激酶（PI3K）和蛋白激酶 B（Akt）水平显著降低。与正常对照组相比，阿托伐他汀组 TC 和 TNF-α水平升高。相反，治疗组大鼠的骨小梁形态改善，结构更加紧密，骨髓腔更小，BALP、BGP、TC、TNF-α和 RANKL 水平降低。此外，与模型组相比，治疗组大鼠骨组织中 E2、BMD、OPG、PI3K 和 Akt 水平升高。与阿托伐他汀组相比，治疗组大鼠 TC 和 TNF-α水平降低。生物力学分析表明，荒漠肉苁蓉给药后，与模型组相比，治疗组大鼠体质量减轻，股骨最终和骨折载荷增加，骨结构更致密，骨髓腔更小，骨膜排列改变。
本研究表明，荒漠肉苁蓉对预防和治疗绝经后高脂性骨质疏松症大鼠具有显著疗效。
