KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine, Daejeon 305-811, Republic of Korea.
J Ethnopharmacol. 2013 Mar 7;146(1):83-9. doi: 10.1016/j.jep.2012.11.037. Epub 2012 Dec 5.
Yijung-tang (YJ), a traditional Asian medicine, is used to treat various diseases. However, its anti-osteoporotic effect and mechanism of action remain unclear. The aim of the present study was to evaluate the anti-osteoporotic effect of YJ in ovariectomized (OVX) rats.
Sprague-Dawley rats were divided into five groups as follows: sham-operated, ovariectomized (OVX), OVX rats treated with 100 g/kg/day 17-estradiol, and OVX rats treated with 0.3 and 1.0 g/kg/day YJ for 12 weeks. Trabecular bone mineral density (BMD) and bone microarchitecture were evaluated by microcomputed tomography. The effects of YJ on osteoblast and osteoclast formation were also investigated in an in vitro model using primary murine bone marrow-derived macrophages and murine calvarial preosteoblasts. mRNA expression of osteoclast differentiation-related genes was measured by real-time quantitative reverse transcription-polymerase chain reaction. Activation of the mitogen-activated protein kinases and nuclear factor-κB (NF-κB) were determined by Western blot.
The decrease of BMD and destruction of bone microarchitecture were significantly reduced in the OVX-induced osteoporosis rat model after 12 weeks of YJ treatment. The anti-osteoporotic effect of YJ on bone loss was due to inhibition of osteoclast differentiation through down-regulation of the NF-κB pathway. In addition, YJ suppressed the induction of nuclear factor of activated T-cells, cytoplasmic 1 and c-Fos following receptor activator of nuclear factor kappa-B ligand stimulation.
These results suggest that YJ possess potent anti-osteoporotic activity in OVX rats and may be a useful remedy for the treatment of postmenopausal osteoporosis.
益宫汤（YJ）是一种传统的亚洲药物，用于治疗各种疾病。然而，其抗骨质疏松作用及其作用机制尚不清楚。本研究旨在评估 YJ 对去卵巢（OVX）大鼠的抗骨质疏松作用。
将 Sprague-Dawley 大鼠分为五组：假手术组、去卵巢组（OVX）、去卵巢组给予 100 g/kg/天 17-雌二醇治疗组、去卵巢组给予 0.3 和 1.0 g/kg/天 YJ 治疗组，共 12 周。通过微计算机断层扫描评估小梁骨骨密度（BMD）和骨微结构。还通过使用原代鼠骨髓来源的巨噬细胞和鼠颅骨前成骨细胞的体外模型研究了 YJ 对成骨细胞和破骨细胞形成的影响。通过实时定量逆转录聚合酶链反应测量破骨细胞分化相关基因的 mRNA 表达。通过 Western blot 测定丝裂原激活的蛋白激酶和核因子-κB（NF-κB）的激活。
12 周 YJ 治疗后，OVX 诱导的骨质疏松大鼠模型中 BMD 的降低和骨微结构的破坏明显减少。YJ 对骨丢失的抗骨质疏松作用是由于通过下调 NF-κB 通路抑制破骨细胞分化所致。此外，YJ 抑制了核因子活化 T 细胞细胞质 1 和 c-Fos 的诱导，受体激活核因子 kappa-B 配体刺激后。
这些结果表明，YJ 对 OVX 大鼠具有强大的抗骨质疏松活性，可能是治疗绝经后骨质疏松症的有效方法。
